Ammonia regulates chicken tracheal cell necroptosis via ...
2020-3-15 · The trachea tissues were cut into several 1-mm 3 pieces, rinsed with saline, and fixed in 2.5 % glutaraldehyde solution with phosphate for 3 h at 4℃. The tissues were then washed three times with phosphate-buffered saline (v/v, pH 7.2); after the last rinse, the tissue pieces were placed in a 1 % osmium tetroxide fixative for 1.5 h followed by dehydration with successively …
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