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jiebaikeli for haemophilus parasuis disease

  • Haemophilus parasuis (Glasser’s Disease) | Iowa State ...

    2016-5-18 · Haemophilus parasuis in BALF at necropsy (days 25 or 35) Figure 5.5. Average levels of IgA antibodies optical density (OD) against 126 Haemophilus parasuis in BALF at necropsy (days 25 or 35) Figure 5.6. Average number of Haemophilus parasuis-specific 127 interferon gamma (IFN-γ) spot forming units (SFU) per 106 cells by day

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  • Glaesserella parasuis – Swine Diseases

    Haemophilus parasuis is a common colonizer of the upper respiratory tract of swine and frequently causes disease, especially in weaner pigs. To date, limited epidemiological data was available for H. parasuis from healthy pigs, which might be carriers of potential pathogenic strains.

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  • The effect of antibiotic treatment on Haemophilus

    Glässer's disease, which is caused by Haemophilus parasuis, is a major threat to swine throughout the world. At present, the predominant method of controlling this disease is through vaccination with an inactivated vaccine, which has many limitations. For example, there is no available method to dif …

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  • Investigation of Haemophilus parasuis from healthy

    2020-8-20 · Glässers Disease is caused by the bacterium Haemophilus parasuis, a small organism, of which there are at least fifteen different types. It is ubiquitous, found throughout the world and is present even in high health herds. If such herds are set up using SPF or MEW techniques and are free from Hps it can be devastating when they first become ...

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  • Haemophilus parasuis vaccines - PubMed

    2020-10-13 · Haemophilus parasuis infection in swine is connmonly referred to as Glasser's disease. Glasser described polyserositis and polyarthritis in young swine associated with a small gram negative organism in 1910 Since that time, H. parasuis infection has been regarded as a sporadic, stress associated disease

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  • Glässers disease (Haemophilus parasuis) | The Pig Site

    Inactivated bacterium for H. parasuis is a traditional vaccine that can elicit efficient protection against homologous challenges. The objective of this study was to screen for the adjuvant-enhanced immune effect of trivalent inactivated H. parasuis serovars 4, 5 and 12 (prevalent serovars in China) vaccines against Glässer's disease.

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  • Pathogenesis of Haemophilus parasuis infection in swine

    Haemophilus parasuis is one kind of nonhaemolytic and nicotinamide adenine dinucleotide (NAD)-dependent Gram-negative bacteria. It is the causative pathogen of Gl€asser disease, which is characterized by fibrinous poly-serositis, meningitis and arthritis (Amano et al. 1994). Other related inflammatory manifestations, such as acute

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  • First comparison of adjuvant for trivalent inactivated ...

    2019-8-29 · Haemophilus parasuis is a major bacterial pathogen, causing polyserositis, polyarthritis, and meningitis—Glässer disease.16 To date, at least 15 serovars have been recognized with a varying ability to induce severe disease. Serovars 1, 5, 10, and 12–14 are the most virulent serovars. 6 Haemophilus parasuis culture is routinely used for diagnosis. The bacteria

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  • Haemophilus parasuis (Glasser’s Disease) | Iowa State ...

    2021-7-17 · Then by 1976, definitive taxonomic studies resulted in the present name, Haemophilus parasuis. The importance of Glasser’s disease has increased with expansion of the swine raising industry. Etiology. Haemophilus parasuis is a small, pleomorphic, and fastidious, Gram-negative rod. It can be grown on blood agar, as satellite colonies, near a ...

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  • Glässers disease (Haemophilus parasuis) | The Pig Site

    2020-8-20 · Glässers Disease is caused by the bacterium Haemophilus parasuis, a small organism, of which there are at least fifteen different types. It is ubiquitous, found throughout the world and is present even in high health herds. If such herds are set up using SPF or MEW techniques and are free from Hps ...

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  • Haemophilus parasuis - an overview | ScienceDirect

    Mark G. Papich DVM, MS, DACVCP, in Saunders Handbook of Veterinary Drugs (Fourth Edition), 2016 Indications and clinical uses. Ceftiofur crystalline-free acid is indicated for treatment of swine respiratory disease (SRD) caused by A. pleuropneumoniae, P. multocida, S. choleraesuis, Haemophilus parasuis, and Streptococcus suis. In cattle, it is used for treatment of BRD caused by Mannheimia ...

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  • Investigation of Haemophilus parasuis from healthy

    2019-4-1 · Haemophilus parasuis is a common colonizer of the upper respiratory tract of swine and frequently causes disease, especially in weaner pigs. To date, limited epidemiological data was available for H. parasuis from healthy pigs, which might be carriers of potential pathogenic strains. In this study, from September 2016 to October 2017, we investigated the prevalence and characteristics of H ...

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  • Evidence for Establishing the Clinical Breakpoint of ...

    2021-1-22 · Haemophilus parasuis is a commensal gram-negative bacterium of the upper respiratory tract. It can invade the body in particular disease conditions. ... against H. parasuis disease from various MICs and adapted a novel algorithm to estimate the COCL. Finally, we attempted to establish the CBP of cefquinome against H. parasuis for

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  • Effect of enrofloxacin on Haemophilus parasuis

    2017-2-1 · Haemophilus parasuis, the causative agent of Glasser’s disease, is a pathogen that colonizes the upper respiratory tract (URT) of pigs, invades the bloodstream and causes polyserositis. Because antimicrobials are highly effective against H. parasuis, we hypothesized that they could have a detrimental effect on the establishment of an immune response if given at the time of URT colonization.

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  • ORIGINAL ARTICLE Validation of a real-time PCR for ...

    Validation of a real-time PCR for Haemophilus parasuis C. Turni, M. Pyke and P.J. Blackall Animal Research Institute, Queensland Primary Industries and Fisheries, Yeerongpilly, Qld, Australia Introduction In 1906, a disease of young pigs associated with poly-serositis and arthritis was described by Karl Gla¨sser (Sutherland and Simmons 1947).

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  • A robust PCR for the differentiation of potential virulent ...

    2017-8-26 · Background: Haemophilus parasuis is the etiological agent of Glässer’s disease in swine. H. parasuis comprises strains with heterogeneous virulence capacity, from non-virulent to highly virulent. Determination of the pathogenic potential of the strains is important for diagnosis and disease control.

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  • Construction and Application of MALDI-TOF Mass ...

    2021-4-15 · Haemophilus parasuis (H. parasuis) disease, also known as Glässer’s disease [1], is induced by H. parasuis, leading to the increase of body temperature, joint swelling, dyspnea, polyserositis, and arthritis in pigs [2]. It is an infec-tious disease with a high mortality rate, seriously endanger-

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  • HtrA Is Important for Stress Resistance and Virulence in ...

    HtrA Is Important for Stress Resistance and Virulence in Haemophilus parasuis Luhua Zhang, aYing Li, Yiping Wen, aGee W. Lau,b Xiaobo Huang, Rui Wu, Qigui Yan,a,c Yong Huang, aQin Zhao, Xiaoping Ma,a Xintian Wen,a Sanjie Caoa,c ResearchCenterofSwineDisease,CollegeofVeterinaryMedicine,SichuanAgriculturalUniversity,Chengdu,Chinaa;DepartmentofPathobiology,UniversityofIllinoisat

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  • Effect of enrofloxacin on Haemophilus parasuis

    Haemophilus parasuis, the causative agent of Glasser's disease, is a pathogen that colonizes the upper respiratory tract (URT) of pigs, invades the bloodstream and causes polyserositis. Because antimicrobials are highly effective against H. parasuis, we hypothesized that they could have a detrimental effect on the establishment of an immune ...

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  • Complete Genome Sequence of Haemophilus

    Haemophilus parasuis is the causative agent of Glässer's disease, which produces big losses in swine populations worldwide. H. parasuis SH0165, belonging to the dominant serovar 5 in China, is a clinically isolated strain with high-level virulence. Here, we report the …

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  • Canada: Serotyping of Haemophilus parasuis field

    2017-8-1 · Haemophilus parasuis, the etiological agent of Glässer’s disease, is one of the most important bacterial pathogens of swine ().It usually causes polyserositis, but infection may also result in arthritis, meningitis, and septicemia (sudden death) ().Haemophilus parasuis also contributes to porcine respiratory disease complex, one of the leading causes of mortality in grower-finisher pigs ...

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  • Enhancement of Haemophilus parasuis serovar 5 yields by ...

    Haemophilus parasuis is one kind of nonhaemolytic and nicotinamide adenine dinucleotide (NAD)-dependent Gram-negative bacteria. It is the causative pathogen of Gl€asser disease, which is characterized by fibrinous poly-serositis, meningitis and arthritis (Amano et al. 1994). Other related inflammatory manifestations, such as acute

    Get Price
  • Differences in susceptibility to Haemophilus parasuis ...

    Haemophilus parasuis, a Gram-negative bacterium, is one of the earliest and most prevalent colonizers of piglets in the farrowing house and a common isolate from nasal secretions in pigs (3).

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  • Development of Serotype-Specific PCR Assays for

    2017-10-24 · The bacterium Haemophilus parasuis is the specific pathogenic cause of Glässer's disease in swine. Fifteen serotypes of H. parasuis have been reported. A method to serotype H. parasuis isolates accurately would help to prevent and control Glässer's disease outbreaks through appropriate vaccination and to understand the epidemiology in specific geographic areas. However, according to ...

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  • First comparison of adjuvant for trivalent inactivated ...

    2015-12-15 · Inactivated bacterium for H. parasuis is a traditional vaccine that can elicit efficient protection against homologous challenges. The objective of this study was to screen for the adjuvant-enhanced immune effect of trivalent inactivated H. parasuis serovars 4, 5 and 12 (prevalent serovars in China) vaccines against Glässer's disease.

    Get Price
  • Evidence for Establishing the Clinical Breakpoint of ...

    2021-1-22 · Haemophilus parasuis is a commensal gram-negative bacterium of the upper respiratory tract. It can invade the body in particular disease conditions. ... against H. parasuis disease from various MICs and adapted a novel algorithm to estimate the COCL. Finally, we attempted to establish the CBP of cefquinome against H. parasuis for

    Get Price
  • Construction and Application of MALDI-TOF Mass ...

    2021-4-15 · Haemophilus parasuis (H. parasuis) disease, also known as Glässer’s disease [1], is induced by H. parasuis, leading to the increase of body temperature, joint swelling, dyspnea, polyserositis, and arthritis in pigs [2]. It is an infec-tious disease with a high mortality rate, seriously endanger-

    Get Price
  • Application of protein profiling of virulent Haemophilus ...

    Moreno et al. – Protein profiling of virulent Haemophilus parasuis J Infect Dev Ctries 2016; 10(6):678-681. 680 Each sample was distributed over six spots and measured four times, resulting in a ...

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  • Pathogenesis of Haemophilus parasuis Infection: Update

    2020-10-13 · Pathogenesis of Haemophilus parasuis Infection: Update of Current Research Abstract Haemophilus parasuis has traditionally been considered a sporadic stress associated disease of young swine in conventional herds.12 In recent years there has been renewed interest in H. parasuis infection for several reasons.

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  • Construction and Application of MALDI-TOF Mass ...

    2021-4-15 · Haemophilus parasuis (H. parasuis) disease, also known as Glässer's disease , is induced by H. parasuis, leading to the increase of body temperature, joint swelling, dyspnea, polyserositis, and arthritis in pigs . It is an infectious disease with a high mortality rate, seriously endangering the health of piglets and young pigs.

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  • Autogenous sow vaccination for Glasser’s disease in

    2009-3-2 · Glasser’s disease in their weaner pigs. Sys-tem A or B supplied 4000 or 7000 weaned pigs weekly to their respective single-site nursery facilities. Suspect serosal lesions were cultured for Haemophilus parasuis, with serotyping and genotyping for tbpA and tbpB transferrin-binding genes. Most cultured H parasuis were either serotype

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  • Development of Serotype-Specific PCR Assays for

    2017-10-24 · The bacterium Haemophilus parasuis is the specific pathogenic cause of Glässer's disease in swine. Fifteen serotypes of H. parasuis have been reported. A method to serotype H. parasuis isolates accurately would help to prevent and control Glässer's disease outbreaks through appropriate vaccination and to understand the epidemiology in specific geographic areas. However, according to ...

    Get Price
  • Haemophilus parasuis VtaA2 is involved in adhesion

    2019-9-23 · Haemophilus parasuis is part of the microbiota of the upper respiratory tract in swine. However, virulent strains can cause a systemic disease known as Glässer’s disease. Several virulence factors have been described in H. parasuis including the virulence-associated trimeric autotransporters (VtaAs). VtaA2 is up-regulated during infection and is only found in virulent strains.

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  • First comparison of adjuvant for trivalent inactivated ...

    2015-12-15 · Inactivated bacterium for H. parasuis is a traditional vaccine that can elicit efficient protection against homologous challenges. The objective of this study was to screen for the adjuvant-enhanced immune effect of trivalent inactivated H. parasuis serovars 4, 5 and 12 (prevalent serovars in China) vaccines against Glässer's disease.

    Get Price
  • Transcription analysis on response of porcine alveolar ...

    2012-2-13 · BACKGROUND: Haemophilus parasuis (H. parasuis) is the etiological agent of Glässer's disease in pigs. Currently, the molecular basis of this infection is largely unknown. The innate immune response is the first line of defense against the infectious disease. Systematical analysis on host innate immune response to the infection is important for ...

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  • Effect of enrofloxacin on Haemophilus parasuis

    2017-3-10 · Haemophilus parasuis, the causative agent of Glasser's disease, is a pathogen that colonizes the upper respiratory tract (URT) of pigs, invades the bloodstream and causes polyserositis.Because antimicrobials are highly effective against H. parasuis, we hypothesized that they could have a detrimental effect on the establishment of an immune response if given at the time of …

    Get Price
  • Glässer Disease in Pigs - Generalized Conditions ...

    Glässer disease is caused by infection with Glaesserella (Haemophilus) parasuis. The most common form is characterized by fibrinous polyserositis and polyarthritis, but septicemia with sudden death and bronchopneumonia also can occur. Diagnosis is based on clinical signs and bacterial isolation or PCR. Antibiotic treatment remains the first ...

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  • Glässer's Disease - Diseases of Swine - Wiley Online

    2019-3-29 · Glässer's disease occurs in swine populations around the world irrespective of health status, and outbreaks of this economically significant disease may be linked to stress-associated events including coinfections and moving and commingling animals. Haemophilus parasuis strains are heterogeneous in phenotypic and genotypic traits, including ...

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  • “Pathotyping” Multiplex PCR Assay for Haemophilus

    2017-8-23 · Haemophilus parasuis is a diverse bacterial species that is found in the upper respiratory tracts of pigs and can also cause Glässer's disease and pneumonia. A previous pangenome study of H. parasuis identified 48 genes that were associated with clinical disease. Here, we describe the development of a generalized linear model (termed a pathotyping model) to predict the potential …

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  • Validation of a real‐time PCR for Haemophilus

    2010-3-10 · Introduction. In 1906, a disease of young pigs associated with polyserositis and arthritis was described by Karl Glässer (Sutherland and Simmons 1947).This disease is now known as Glässer’s disease and is caused by the bacterium Haemophilus parasuis (Oliveira and Pijoan 2004). Haemophilus parasuis is a fastidious and delicate organism, characteristics which cause problems in the diagnosis ...

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  • Special Issue 'About Glaesserella (Haemophilus)

    Glaesserella ( Haemophilus) parasuis is one of the secondary bacterial infectious agents frequently detected in PRDC which, although usually being part of the commensal microbiota in the upper respiratory tract of pigs, can gain access to the bloodstream and result in a sepsis named Glässer’s disease, characterized by pneumonia and ...

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  • Haemophilus parasuis VtaA2 is involved in adhesion

    2019-9-23 · Haemophilus parasuis is part of the microbiota of the upper respiratory tract in swine. However, virulent strains can cause a systemic disease known as Glässer’s disease. Several virulence factors have been described in H. parasuis including the virulence-associated trimeric autotransporters (VtaAs). VtaA2 is up-regulated during infection and is only found in virulent strains.

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  • Molecular serotyping of clinical strains of

    2019-5-23 · Introduction. Haemophilus (Glaesserella) parasuis is a pleomorphic, NAD-dependent, Gram-negative bacterium of the Pasteurellaceae family (Biberstein & White, 1969). H. parasuis normally resides in the upper respiratory tract of pigs but can also cause a systemic infection named Glässer’s disease (GD), which is characterized by pneumonia, fibrinous polyserositis, polyarthritis and meningitis ...

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  • Glasser's disease and prevalence of subclinical

    The prevalence of the causative organism, Haemophilus parasuis, was determined for 19 SPF herds in Ontario classified as “Excellent” under the Ontario Swine Herd Health Policy. Nasal swabs from two-month-old pigs were cultured on chocolate agar containing 1.5 μg/mL lincomycin, 5 μg/mL bacitracin, and 0.1 μg/mL crystal violet.

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  • RESEARCH ARTICLE Open Access Differences in

    2017-8-22 · disease caused by these serovars. Keywords: Haemophilus parasuis, Glässer’s disease, AOC-45 cells, Adherence, Invasion Background Glässer’s disease is a swine disease caused by a pleo-morphic nicotinamide adenine dinucleotide-dependent Gram-negative rod of the family Pasteurellaceae known as Haemophilus parasuis [1]. The primary signs of this

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  • Altered indirect hemagglutination method for easy ...

    Glässer’s disease is an emergent bacterial disease that affects swine husbandries worldwide causing important economic losses. The aetiological agent, Haemophilus parasuis, is currently divided in fifteen serovars but an increasing number of non-typeable serovars have been reported. Indirect

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  • Biofilm formation by virulent and non-virulent strains

    2014-11-27 · Haemophilus parasuis is a commensal bacterium of the upper respiratory tract of healthy pigs. It is also the etiological agent of Glässer’s disease, a systemic disease characterized by polyarthritis, fibrinous polyserositis and meningitis, which causes high morbidity and mortality in piglets. The aim of this study was to evaluate biofilm formation by well-characterized virulent and non ...

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  • Development and application of an antibody detection

    2020-1-6 · Haemophilus parasuis, a member of the family Pasteur-ellaceae, constitutes an early colonizer of the swine upper respiratory tract [1]. H. parasuis causes swine Glässer’s disease, which is characterized by fibrinous polyserositis, polyarthritis, and meningitis [2]. Glässer’s disease is distributed worldwide, affecting 1–4month-

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  • HtrA Is Important for Stress Resistance and Virulence

    2017-11-21 · BACKGROUND: Postweaning multisystemic wasting syndrome (PMWS) is an emerging disease in swine. Pigs with PMWS are often infected with a variety of other pathogens, including bacteria, viruses and mycoplasm, in addition to porcine circovirus type 2 (PCV2). PCV2 and Haemophilus parasuis serovar 4 (HPS4) coinfection remain epidemic in China.

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  • Coinfection with Haemophilus parasuis serovar 4

    Glässer’s disease is an emergent bacterial disease that affects swine husbandries worldwide causing important economic losses. The aetiological agent, Haemophilus parasuis, is currently divided in fifteen serovars but an increasing number of non-typeable serovars have been reported. Indirect

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  • Altered indirect hemagglutination method for easy ...

    2011-10-15 · Haemophilus parasuis is a member of the family Pasteurellaceae and is the etiologic agent of Glässer’s disease in pigs, a systemic syndrome associated with only a subset of isolates. The genetic basis for virulence and systemic spread of particular H. parasuis isolates is currently unknown. Strain 29755 is an invasive isolate that has long been used in the study of Glässer’s disease.

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  • Genome sequence of Haemophilus parasuis strain

    2009-8-19 · Aims: To validate a real-time PCR test for the diagnosis of Glässer’s disease, a major pig disease caused by Haemophilus parasuis. Methods and Results: The specificity of a real-time PCR amplifying the inf B gene was validated with 68 H. parasuis …

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  • Prevalence and seroepidemiology of Haemophilus

    Background Haemophilus parasuis is the etiological agent of Glässer’s disease, and causes severe economic losses in the swine industry. Serovar classification is intended as an indicator of virulence and pathotype and is also crucial for vaccination programs and vaccine development.

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  • Validation of a real-time PCR for Haemophilus

    2021-6-25 · Haemophilus parasuis are antigenic proteins expressed in vivo Alex Olvera, Sonia Pina, Marta Pérez-Simó, Simone Oliveira, Albert Bensaid ... Vaccination has been widely used to control the disease, although there is a lack of extended cross-protection. Trimeric autotransporters, a family of surface exposed proteins implicated in host-pathogen ...

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  • Molecular serotyping of Haemophilus parasuis

    162 Pigs diagnosed with Haemophilus parasuis disease (HPS) were selected and randomly divided into 9 groups, with 18 pigs in each group. Treatments included amoxicillin nicotinate mixed drink high-dose group, medium-dose group and low-dose group, amoxicillin nicotinate intramuscular injection high-dose group, medium-dose group and low-dose group, ampicillin mixed drink group and intramuscular...

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  • Virulence-associated trimeric autotransporters of ...

    Gla¨sser’s disease, characterized by serofibrinous pleurisy, pericarditis, peritonitis, arthritis and meningitis, is caused by Haemophilus parasuis (Oliveira and Pijoan 2004). This organism is also considered as a common inhabitant of the upper respiratory tract of healthy pigs, but some strains can disseminate and produce severe systemic dis-

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  • Diagnosis of Haemophilus parasuis in affected herds

    Oliveira S, Pijoan C. Diagnosis of Haemophilus parasuis in affected herds and use of epidemiological data to control disease. J Swine Health Prod. 2002;10 (5):221-225. H aemophilus parasuis is a commensal organism in the upper respiratory tract of pigs. 1 This organism is particularly interesting due to its ability to invade the host and cause ...

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  • Validation of a real‐time PCR for Haemophilus

    2010-3-10 · Aims: To validate a real-time PCR test for the diagnosis of Glässer’s disease, a major pig disease caused by Haemophilus parasuis. Methods and Results: The specificity of a real-time PCR amplifying the inf B gene was validated with 68 H. parasuis isolates and 36 strains of closely related species. As well, 239 samples of DNA from tissues and fluids of 16 experimentally challenged animals ...

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  • Validation of a real-time PCR for Haemophilus

    2009-8-19 · Aims: To validate a real-time PCR test for the diagnosis of Glässer’s disease, a major pig disease caused by Haemophilus parasuis. Methods and Results: The specificity of a real-time PCR amplifying the inf B gene was validated with 68 H. parasuis …

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  • Prevalence and seroepidemiology of Haemophilus

    Haemophilus parasuis is a bacterium that causes disease in many body systems of pigs, but is also a colonizer of the upper respiratory tract. It may affect all ages but is particularly common in nursery pigs, growers and early finishers. Hill et al. (1996) found H. parasuis in 7.9% of cases of arthritis in pigs under 12 weeks of age. Primarily it causes Glässer’s Disease, a polyserositis ...

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  • Glässer's disease - CABI.org

    2019-8-28 · Haemophilus parasuis. is the causative agent of Glässer’s disease, an inflammatory condition featured by serofibrinous to fibrinopurulent exudates at single or multiple serosal sur-faces (porcine polyserositis and arthritis). 1. Polyarthritis is usually more severe in the atlanto-occipital and large limb joints. 19. Haemophilus parasuis

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  • Fatal infection due to Haemophilus parasuis in a young ...

    Furthermore, what is Glasser's disease? Glasser's disease is caused by a bacterium called Haemophilus parasuis. Acute outbreaks can cause a high mortality, while it is also an important cause of pleurisy found in slaughter pigs. Between farms, spread is generally by the movement of carrier pigs that are not showing clinical signs of disease.

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  • What is Haemophilus Parasuis? - AskingLot.com

    2020-5-26 · Haemophilus parasuis is the etiological agent of Glässer's disease in swine, which associates with severe economic losses in the swine industry worldwide. A real‐time recombinase polymerase amplification assay (real‐time RPA) was developed for direct and rapid detection of H. parasuis basing on the translation‐initiation factor IF2 (infB ) gene.

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  • Development of a recombinase polymerase

    2019-12-3 · Haemophilus parasuis is a commensal pathogen in the swine upper respiratory tract and causes Glässer’s disease. Surveillance, screening for infection, and vaccination response of H. parasuis is hindered by the lack of a rapid antibody detection method. In the present study, a monomeric autotransporter was identified as a novel antigen for developing an indirect ELISA.

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  • Development and application of an antibody

    2019-9-1 · Pathogens Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, 1920 Dayton Avenue, Building 24, Ames, IA 50010. [email protected] Detection of a bacteriophage gene encoding a Mu-like portal protein in Haemophilus parasuis reference strains and field isolates by nested polymerase chain reaction

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  • Pathogenesis of Haemophilus parasuis infection in swine

    2020-10-13 · Haemophilus parasuis infection in swine is connmonly referred to as Glasser's disease. Glasser described polyserositis and polyarthritis in young swine associated with a small gram negative organism in 1910 Since that time, H. parasuis infection has been regarded as a sporadic, stress associated disease

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  • The effect of antibiotic treatment on Haemophilus

    The effect of antibiotic treatment on Haemophilus parasuis colonization, disease and immunity. Authors. Macedo, Nubia. Issue Date 2015-07. Type. Thesis or Dissertation. Abstract. Glasser's disease is an important source of economic losses in commercial swine production systems. The success of controlled exposure in the field suggests that early ...

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  • Validation of a real‐time PCR for Haemophilus

    2010-3-10 · Aims: To validate a real-time PCR test for the diagnosis of Glässer’s disease, a major pig disease caused by Haemophilus parasuis. Methods and Results: The specificity of a real-time PCR amplifying the inf B gene was validated with 68 H. parasuis isolates and 36 strains of closely related species. As well, 239 samples of DNA from tissues and fluids of 16 experimentally challenged animals ...

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  • Establishment of a Successive Markerless Mutation

    2015-5-18 · Haemophilus parasuis, belonging to the family Pasteurellaceae, is the causative agent of Glässer’s disease leading to serious economic losses. In this study, a successive markerless mutation system for H. parasuis using two sequential steps of natural transformation was developed. By the first homologous recombination, the target genes were replaced by a cassette carrying kanamycin ...

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  • Molecular serotyping of clinical strains of

    Haemophilus parasuis is a colonizer of the upper respiratory tract of pigs, although it is better known as the etiological agent of Glässer's disease. Interestingly, several strains can be isolated from a single farm, as determined by both genotyping and serotyping. However, it is not known how an outbreak and the subsequent treatment affect the population of H. parasuis strains.

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  • [PDF] Dynamics of Haemophilus parasuis genotypes

    2019-8-28 · Haemophilus parasuis. is the causative agent of Glässer’s disease, an inflammatory condition featured by serofibrinous to fibrinopurulent exudates at single or multiple serosal sur-faces (porcine polyserositis and arthritis). 1. Polyarthritis is usually more severe in the atlanto-occipital and large limb joints. 19. Haemophilus parasuis

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  • Fatal infection due to Haemophilus parasuis in a young ...

    2017-11-21 · Haemophilus parasuis(HPS), and Actinobacillus pleur-opneumoniae (APP), a variety of viral and bacterial pathogens in the majority of cases (85.0%) [4, 5]. HPS is an important swine pathogen that causes Glässer’s disease, which is characterized by fibrinous …

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  • Coinfection with Haemophilus parasuis serovar 4

    Summary. During development of Glassinord®, a vaccine against Haemophilus parasuis, observations on the immunity against G lässer 's disease were made.. The efficacy of Glassinord® was studied in a sow herd with very low CF titres against H. parasuis, by vaccination of part of the weaners, leaving the others as non-vaccinated controls.After transfer to a fattening farm 11 of 26 controls (42 ...

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  • Prevention of Glässer's Disease through Immunity to ...

    2020-11-17 · Glaesserella parasuis is an important pathogen in swine production. It acts as a primary pathogen in systemic Glässer´s disease and as a secondary pathogen in Porcine Respiratory Disease Complex. In this study, a collection of 308 isolates from carrier animals and individuals with respiratory or Glässer´s disease isolated 2012–2019 in Germany was analysed.

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  • Pathogenesis of Haemophilus parasuis infection in swine

    2020-10-13 · Haemophilus parasuis infection in swine is connmonly referred to as Glasser's disease. Glasser described polyserositis and polyarthritis in young swine associated with a small gram negative organism in 1910 Since that time, H. parasuis infection has been regarded as a sporadic, stress associated disease

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  • Update on the diagnosis of Haemophilus parasuis

    2006-12-18 · Haemophilus parasuis causes Glässer's disease as well as a number of other diseases in pigs. The diagnosis of H. parasuis-associated disease is usually established by clinical signs, pathological findings and bacterial isolation but diagnosis is complicated by the existence of non-virulent strains and the early colonisation of the upper respiratory tract of healthy piglets.

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  • Immunohistochemical Detection of Haemophilus Parasuis ...

    2018-2-21 · Haemophilus parasuis is a small gram-negative, non-motile, rod-shaped bacterium that grows in enriched medium; it requires NAD (V factor) to grow. 3,16 Infec-tion with H. parasuis (Glässer’s disease) is usually manifested as a fibrinopurulent polyserositis.8 How-ever, this bacterium can also cause pneumonia13 and

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  • Research Article Simultaneous Detection of Actinobacillus ...

    Haemophilus parasuis (H. para-suis) is the specific pathogenic cause of Glässer’s disease in swine, which brings about polyserositis in swine, including pleuritis, peritonitistis and arthritis [7]. Both of them are among the most common bacterial causes of porcine respiratory disease, and often

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  • RESEARCH ARTICLE Open Access Transcriptomics of ...

    2018-11-6 · Background: Haemophilus (Glässerella) parasuis is the etiological agent of Glässer’s disease in pigs. Control of this disorder has been traditionally based on bacterins. The search for alternative vaccines has focused mainly on the study of outer membrane proteins. This study investigates the transcriptome of H. (G.) parasuis serovar 5 ...

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  • Prevalence and seroepidemiology of [i]Haemophilus

    2020-1-20 · 22 23 Abstract 24 Haemophilus parasuis, the causative agent of Glässer’s disease, has been reported widely, but 25 seldom is known about its epidemiology in Sichuan province, China. The objective was to reveal 26 the prevalence and distribution of H.parasuis in the area. Widely sampling and isolation was 27 performed initially and following serotyping multiplex PCR (serotyping-mPCR ...

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  • Coinfection with Haemophilus parasuis serovar 4

    2017-11-21 · Haemophilus parasuis(HPS), and Actinobacillus pleur-opneumoniae (APP), a variety of viral and bacterial pathogens in the majority of cases (85.0%) [4, 5]. HPS is an important swine pathogen that causes Glässer’s disease, which is characterized by fibrinous …

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  • Pathogenicity and Immunity Studies of Haemophilus

    1993-6-1 · After intranasal inoculation of SPF pigs with the 7 reference strains of Haemophilus parasuis only the reference strains of serotypes 1 and 5 proved to be pathogenic and were able to produce the lesions characteristic of Glässer’s disease. Even though the reference strains of serotypes 2, 3, 4, 6 and 7 proved to be apathogenic, intranasal inoculation resulted in the development of ...

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  • Serotyping and pathotyping of Glaesserella parasuis ...

    2020-11-17 · Glaesserella parasuis is an important pathogen in swine production. It acts as a primary pathogen in systemic Glässer´s disease and as a secondary pathogen in Porcine Respiratory Disease Complex. In this study, a collection of 308 isolates from carrier animals and individuals with respiratory or Glässer´s disease isolated 2012–2019 in Germany was analysed.

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  • Detection of a putative hemolysin operon, hhdBA, of ...

    2019-8-29 · The aim of the current study was to investigate whether polymerase chain reaction amplification of 16S ribosomal (r)RNA and a putative hemolysin gene operon, hhdBA, can be used to monitor live pigs...

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  • Diagnosis of Haemophilus parasuis in affected herds

    Oliveira S, Pijoan C. Diagnosis of Haemophilus parasuis in affected herds and use of epidemiological data to control disease. J Swine Health Prod. 2002;10 (5):221-225. H aemophilus parasuis is a commensal organism in the upper respiratory tract of pigs. 1 This organism is particularly interesting due to its ability to invade the host and cause ...

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  • Characterization of Chinese Haemophilus parasuis

    2016-12-22 · Introduction. Haemophilus parasuis, the causative agent of Glässer’s disease in pigs, has multiple clinical manifestations, including pneumonia, meningitis, arthritis, polyserositis, and septicemia [1–4].Glässer’s disease outbreaks are seriously damaging to pigs and cause devastating economic losses to the swine industry worldwide, either on their own, or when co-infections with other ...

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  • Interactions of Haemophilus parasuis and its LOS with ...

    2021-7-2 · Abstract Haemophilus parasuis is a swine pathogen that causes Glässer s disease, which is characterized bypolyserositisandmeningitis.Thepathogenesisofthe H.parasuis infectionispoorlyunderstood.Tocause meningitis, H. parasuis has to cross the blood-brain barrier (BBB) to gain access to the central nervous system (CNS).

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  • Haemophilus parasuis and its role in swine disease.

    Clinical signs of respiratory distress associated with H. parasuis, the principal finding upon necropsy is reviewed. Bacterial isolation of H. parasuis is necessary to confirm a diagnosis of Glasser's disease. Treatment and prevention by vaccination are discussed.

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  • Molecular serotyping of Haemophilus parasuis isolated

    2018-11-29 · Haemophilus parasuis, a part of normal upper respiratory microbiota, is the etiological agent of Glässer’s disease which induces sudden death, polyserositis, polyarthritis, meningitis and poor production performance, resulting in severe economic losses in the

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  • RESEARCH ARTICLE Open Access Comparison of

    2017-8-23 · Keywords: Haemophilus parasuis, RAPD, SDS-PAGE Background Haemophilus parasuis causes Glässer’s disease in pigs, with symptoms of fibrinous polyserositis, pericarditis, polyarthritis, and meningitis [1]. H. parasuis also causes septicemia and pneumonia without polyserositis and can be isolated from nasal passages of healthy swine.

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  • Map-Based Comparative Genomic Analysis of Virulent ...

    2015-3-31 · Haemophilus parasuis is a commensal bacterium of the upper respiratory tract of healthy pigs. However, in conjunction with viral infections in immunocompromised animals H. parasuis can transform into a pathogen that is responsible for causing Glasser’s disease which is typically

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  • Effect of enrofloxacin on Haemophilus parasuis

    Haemophilus parasuis, the causative agent of Glasser's disease, is a pathogen that colonizes the upper respiratory tract (URT) of pigs, invades the bloodstream and causes polyserositis. Because antimicrobials are highly effective against H. parasuis, we hypothesized that they could have a detrimental effect on the establishment of an immune response if given at the time of URT colonization.

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  • Transcriptomics of Haemophilus (Glässerella) parasuis ...

    Haemophilus (Glässerella) parasuis is the etiological agent of Glässer’s disease in pigs. Control of this disorder has been traditionally based on bacterins. The search for alternative vaccines has focused mainly on the study of outer membrane proteins. This study investigates the transcriptome of H. (G.) parasuis serovar 5 subjected to in vitro conditions mimicking to those existing ...

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  • Molecular serotyping of clinical strains of Haemophilus ...

    2019-5-20 · Haemophilus (Glaesserella) parasuis is a pleomorphic, NAD-dependent, Gram-negative bacterium of the Pasteurellaceae family (Biberstein & White, 1969). H. parasuis normally resides in the upper respiratory tract of pigs but can also cause a systemic infection named Glässer’s disease (GD), which is characterized by pneumonia, fibrinous ...

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