A Protocol to Engineer Bacteriophages for Live-Cell ...
2020-9-18 · Plate 100 μL of this mixture onto LB + kanamycin plates to select for kanamycin resistant phage lysogens. 54. Incubate plates at 37°C for 16 h. Note: If working with a temperature sensitive mutant of cI such as cI857, then this step should be performed at 30°C. 55. Pick several colonies with an inoculating loop and resuspend in 20 μL of H 2 ...
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