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qizhenzengmiankeli granules for pseudorabies using method

  • Pseudorabies virus infection inhibits stress granules ...

    2020-8-1 · Pseudorabies virus (PRV) is one of the most notorious pathogens in the global pig industry. During infection, viruses may evolve various strategies, such as modulating stress granules (SGs) formation, to create an optimal surroundings for viral replication. However, the interplay between PRV infection and SGs formation remains largely unknown.

    Get Price
  • Function of Torsin AAA+ ATPases in Pseudorabies

    2020-3-15 · 2018年发表SCI论文-华中农业大学农业微生物学国家重点实验室. 1. Lin Da#, Hong Ping#, Zhang Siheng, Xu Weize, Jamal M, Yan Keji, Lei Yingying, Li Liang, Ruan Yijun, Fu Zhen F., Li Guoliang*, Cao Gang*, Digestion-ligation-only Hi-C is an efficient and cost-effective method for chromosome conformation capture, Nat Genet, 2018,50 (5):754. 2.

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  • Golgi staining-like retrograde labeling of brain circuits ...

    Cells were transfected with 1 µg of plasmid DNA using polyethylenimine (PEI) , and harvested 24 h post transfection by scraping into the medium, pelleted, washed twice with phosphate-buffered saline (PBS) and lysed in SDS-containing sample buffer (0.13 M Tris-HCl, pH 6.8; 4% SDS; 20% glycerin; 0.01% bromophenol blue; 10% 2-mercaptoethanol). Here we used PEI transfection, instead of calcium phosphate-precipitation method…

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  • Quantitative electron microscope studies on the

    2020-10-1 · The gold standard to detect the fine details of neuronal dendritic arborization is still the silver staining technique that has been used by Cajal, called Golgi metallic impregnation method. This method or its updates (Rapid Golgi staining, Golgi-Cox method) allows staining a limited number of neurons in their entirety, in a still unexplained random manner.

    Get Price
  • Detection of Prion Protein in Formalin-Fixed Brain by ...

    The appearance of the fine-grained aggre- gates appears to be related to the growth of virus in the nuclei. The aggregates resemble those found in cells infected with other viruses including varicella (Tournier et al., 1957), polyoma (Bernhard et al., 1959), EMC (Hinz et al., 1962), SV40 (Gran- boulan et al., 1963), and pseudorabies (Felluga, 1963).

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  • A soluble form of human nectin-2 impairs exocrine ...

    2018-2-23 · ing. 1 This novel method of antigen enhancement, termed hydrated autoclaving, was originally devised to improve the immunoreactivity of certain brain proteins. 10 Hydrated au-toclaving has not been used to detect other antigens. How-ever, antigen enhancement is believed to result from heat denaturation of the protein, and the same mechanism has

    Get Price
  • Progress and Perspective of Antiviral Protective

    2016-3-1 · Transgenic mice expressing hNectin-2Ig were generated as described previously , by microinjection method of the transgenic fragment containing the CAG promoter (cytomegalovirus IE enhancer and chicken ß-actin promoter), the hNectin-2Ig gene, and the rabbit ß-globin polyA signal. To identify transgenic founders, genomic DNA was prepared by using the FTA elute micro cards (Whatman) and PCR was performed using …

    Get Price
  • The development of antibodies in rabbits and cattle ...

    2020-6-23 · The average particle size of Ag NPs is an important factor that affects its antiviral ability. For example, Krzyzowska et al. report the synthesis of Ag NPs with an average particle size of 33 nm, prepared by chemical reduction method, using tannic acid as surface ligands. This Ag NPs couls effectively control the herpes simplex virus type 2 (HSV-2) infection in mice by inhibiting the adhesion …

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  • Efficient Retrograde Transport of Pseudorabies Virus ...

    After replicating in epithelial cells, alphaherpesviruses such as pseudorabies virus (PRV) invade axons of peripheral nervous system neurons and undergo retrograde transport toward the distant cell bodies. Although several viral proteins engage molecular motors to facilitate transport, the initial steps and neuronal responses to infection are poorly understood. Using compartmented neuron ...

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  • Function of Torsin AAA+ ATPases in Pseudorabies

    Newly assembled herpesvirus nucleocapsids traverse the intact nuclear envelope by a vesicle-mediated nucleo-cytoplasmic transport for final virion maturation in the cytoplasm. For this, they bud at the inner nuclear membrane resulting in primary enveloped particles in the perinuclear space (PNS) followed by fusion of the primary envelope with the outer nuclear membrane (ONM).

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  • Adrenergic innervation of the human testis,

    Spinal neurons involved in the control of the seminal vesicles: a transsynaptic labeling study using pseudorabies virus in rats. Sun XQ, Xu C, Leclerc P, Benoît G, Giuliano F, Droupy S. Neuroscience, 158(2):786-797, 10 Oct 2008 Cited by: 14 articles | PMID: 18977414

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  • Ex vivo infection of human embryonic spinal cord

    2010-5-29 · Genetically modified pseudorabies virus (Prv) proved suitable for the delivery of foreign genes to rodent embryonic neurons ex vivo and maintaining foreign gene expression after transplantation into spinal cord in our earlier study. The question arose of whether human embryonic neurons, which are known to be more resistant to Prv, could also be infected with a mutant Prv.

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  • Quantitative electron microscope studies on the

    VIROLOGY 24, 523-538 (1964) Quantitative Electron Microscope Studies on the Growth of Herpes Virus Using the Techniques of Negative Staining and Ultramicrotomy D. H. WATSON,1 P. WILDY,1 AND W. C. RUSSELL2 Institute of Virology, The University, Glasgow, W.SS., Scotland Accepted July 9, 1964 The morphological differentiation of herpes virus during a single-step growth cycle has been studied by ...

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  • CELLULOLYTIC ENZYMES, NUCLEIC ACIDS ENCODING

    A method for utilizing a cellulase as a nutritional supplement in an animal diet, the method comprising: preparing a nutritional supplement containing a cellulase enzyme comprising at least thirty contiguous amino acids of a polypeptide as set forth in claim 48, or a polypeptide encoded by a nucleic acid as set forth in claim 1; or a ...

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  • Nucleic Acids and Proteins and Methods for Making

    2020-3-15 · Wang Tian, Xiang Yuqiang, Liu Xiaoxiao, Chen Wenli, Hu Yonggang*, A novel fluorimetric method for laccase activities measurement using Amplex Red as substrate, Talanta, 2017,162:143-150. 108. Nie Hailing, Xiao Yujie, Liu Huizhong, He Jinzhi, Chen Wenli*, Huang Qiaoyun*, FleN and FleQ play a synergistic role in regulating lapA and bcs operons in Pseudomonas putida KT2440, Env Microbiol …

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  • HYDROLASES, NUCLEIC ACIDS ENCODING THEM

    A method for whole cell engineering of new or modified phenotypes by using real-time metabolic flux analysis, the method comprising the following steps: (a) making a modified cell by modifying the genetic composition of a cell, wherein the genetic composition is modified by addition to the cell of a nucleic acid comprising a sequence as set ...

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  • Nanotechnology - IOPscience

    2011-11-10 · In one aspect of this second method, step (d) further comprises using ion exchange resins. The kinetically controlled conditions can comprise non-equilibrium conditions resulting in production of an end product having greater than a 2:1 ratio of 1,3-DAG to 2,3-DAG. The composition of step (b) can comprise a fluorogenic fatty acid (FA).

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  • CDC - Diagnosis: Histologic Examination - Rabies

    2010-5-29 · Genetically modified pseudorabies virus (Prv) proved suitable for the delivery of foreign genes to rodent embryonic neurons ex vivo and maintaining foreign gene expression after transplantation into spinal cord in our earlier study. The question arose of whether human embryonic neurons, which are known to be more resistant to Prv, could also be infected with a mutant Prv.

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  • Ex vivo infection of human embryonic spinal cord

    2016-5-23 · Results. Among extracts, 37 L EA was most cytotoxic to Vero cells, followed by 37 L H and 37 L ET, with CC 50 of 218, 833, and >1000 μg/mL, respectively. The cytopathic effect (CPE) and plaque reduction, inhibition, and virucidal assays and the selective index (SI) were employed to determine the effect of the extracts on infectivity and replication of pseudorabies virus (PrV) in Vero cells.

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  • Antiviral and virucidal activities of Duabanga

    2014-9-17 · Here we present a novel tracing technique to stain projection neurons in Golgi-like detail by double viral infection. We used retrograde lentiviral vectors and adeno-associated viral vectors (AAV) to drive “TET-ON/TET-OFF system” in neurons connecting two regions. Using this method, we successfully labeled the corticothalamic (CT) cells of the mouse somatosensory barrel field (S1BF) and ...

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  • Frontiers | Simultaneous visualization of extrinsic and ...

    2018-2-23 · processed by the hydrated autoclaving method. A staining reaction was observed in only 1 sheep from the scrapie-free flock, and in that animal, the immunostained material was limited to sparse, small, intracytoplasmic granules that were present in just a few neurons. Based on this observation, tissues were considered positive for PrP-res only ...

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  • Detection of Prion Protein in Formalin-Fixed Brain by ...

    An improved process for the purification of antibodies from human plasma or other sources is disclosed. The process involves suspension of the antibodies at pH 3.8 to 4.5 followed by addition of caprylic acid and a pH shift to pH 5.0 to 5.2. A precipitate of contaminating proteins, lipids and caprylate forms and is removed, while the majority of the antibodies remain in solution.

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  • USRE43655E1 - Chromatographic method for high

    2020-6-23 · Public health events caused by viruses pose a significant risk to humans worldwide. From December 2019 till now, the rampant novel 2019 coronavirus (SAR-CoV-2) has hugely impacted China and over world. Regarding a commendable means of protection, mask technology is relatively mature, though most of the masks cannot effectively resist the viral infections. The key material of the mask is …

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  • Progress and Perspective of Antiviral Protective

    CXCL9 promotes bone marrow regeneration, increases peripheral white blood cells, and increases survival if administered prior to treatment of a subject with chemotherapeutic drugs such as 5-FU or radiotherapy. Similar effects are obtained by administering an anti-CXCL9 antibody following chemotherapy or radiotherapy. Compositions and methods are presented for the treatment of cancer …

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  • US Patent for Methods for promoting protection and ...

    In one aspect of this second method, step (d) further comprises using ion exchange resins. The kinetically controlled conditions can comprise non-equilibrium conditions resulting in production of an end product having greater than a 2:1 ratio of 1,3-DAG to 2,3-DAG. The composition of step (b) can comprise a fluorogenic fatty acid (FA).

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  • Hydrolases, nucleic acids encoding them and methods

    1993-8-10 · The invention also provides a method for producing a vaccine, comprising inactivating a microorganism or virus in a way which preserves and enhances its immunogenic properties using the method of the invention, vaccines produced by this method, and methods for vaccinating against a disease, comprising immunization with the vaccine of the ...

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  • CDC - Diagnosis: Histologic Examination - Rabies

    2016-1-7 · The virion host shutoff protein (vhs), encoded by the gene UL41, has RNase activity and is the key regulator of the early host shutoff response induced by type 1 herpes simplex virus.Despite low amino acid similarity, the vhs protein of the swine herpesvirus, pseudorabies …

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  • The pseudorabies virus vhs protein cleaves RNA

    2014-9-17 · Using this method, we successfully labeled the corticothalamic (CT) cells of the mouse somatosensory barrel field (S1BF) and motor cortex (M1) in their entirety. We also labeled contra- and ipsilaterally-projecting corticocortical (CC) cells of M1 by targeting contralateral M1 or ipsilateral S1 for retrograde infection.

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  • Simultaneous visualization of extrinsic and intrinsic

    2019-10-29 · The luminal pH of the secretory pathway plays a critical role in the posttranslational modification and sorting of proteins and lipids. The pH of each one of the organelles that constitute the pathway is unique, becoming more acidic as the biosynthetic cargo approaches its destination. The methods used for measurement of pH in the secretory ...

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  • The pH of the Secretory Pathway: Measurement,

    2018-2-23 · processed by the hydrated autoclaving method. A staining reaction was observed in only 1 sheep from the scrapie-free flock, and in that animal, the immunostained material was limited to sparse, small, intracytoplasmic granules that were present in just a few neurons. Based on this observation, tissues were considered positive for PrP-res only ...

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  • Detection of Prion Protein in Formalin-Fixed Brain by ...

    C07K 14/70539. In this subclass non-limiting references (in the sense of paragraph 39 of the Guide to the IPC) may still be displayed in the scheme. C07K 1/00. General methods for the preparation of peptides {, i.e. processes for the organic chemical preparation of peptides or proteins of any length} [2016-08]

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  • CPC Scheme - C07K PEPTIDES

    2019-10-7 · Background. Porcine infectious pleuropneumonia caused by Actinobacillus pleuropneumoniae (App) is one of the most serious infectious diseases in pigs and has brought huge economic losses to the world pig industry. The aim of this trial was to evaluate the effect of enteric-coated tilmicosin granule in the treatment and control of artificial infection of App.

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  • Efficacy of enteric‐coated tilmicosin granules in pigs ...

    stain. Red staining granules in the cytoplasm of nerve cells were first described by Held,13 in 1895, by his erythrosin (iodo-eosin) methylene blue method. He termed them neurosomes. I have obtained satisfactory results from using erythrosin to stain Negri bodies. The Negri body gives a …

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  • blue to a PH 5, I have been able to - JSTOR

    2021. Sun L, Chen H, Ming X, Bo Z, Shin HJ, Jung YS, Qian Y. Porcine Epidemic Diarrhea Virus Infection Induces Caspase-8 mediated G3BP1 Cleavage and Subverts Stress Granules to Promote Viral Replication. Journal of Virology. PMID 33568512 DOI: 10.1128/JVI.02344-20.

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  • Veterinary Microbiology and Preventive Medicine

    Contemporary diagnostic approaches for pseudorabies (Aujeszky's disease) virus based on swine oral fluid specimens, Ting-Yu Cheng. PDF. Generation of site-specific mutations in Mycoplasma, Jeannett Clampitt. PDF. Bacterial succession and specialization within the poultry gastrointestinal tract, Joel Jacob Maki. Theses/Dissertations from 2020 PDF

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  • CRISPR/Cas Technology in Pig-to-Human

    2021-3-21 · 5. Conclusions. The use of the CRISPR/Cas system in genetic engineering has enabled the enormous development of pig-to-human xenotransplantation research. Thanks to this technology, animals with multi-modifications were obtained, enabling the reduction in the primates’ immune reaction to a porcine xenograft.

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  • CPC Scheme - C07K PEPTIDES

    C07K 14/70539. In this subclass non-limiting references (in the sense of paragraph 39 of the Guide to the IPC) may still be displayed in the scheme. C07K 1/00. General methods for the preparation of peptides {, i.e. processes for the organic chemical preparation of peptides or proteins of any length} [2016-08]

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  • Efficacy of enteric‐coated tilmicosin granules in pigs ...

    2019-10-7 · Background. Porcine infectious pleuropneumonia caused by Actinobacillus pleuropneumoniae (App) is one of the most serious infectious diseases in pigs and has brought huge economic losses to the world pig industry. The aim of this trial was to evaluate the effect of enteric-coated tilmicosin granule in the treatment and control of artificial infection of App.

    Get Price
  • Identification of Antigenic and Immunogenic Proteins

    2018-1-1 · Dense granules, micronemes, and rhoptries are secretory organelles in this protozoan . The immunoprotective value of rhoptry protein5 (ROP5) was studied in BALB/c mice using a recombinant form of the protein alone and in combination with rSAG1. rROP5 could induce significant cellular and humoral immune responses ( 5 ).

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  • US Patent for Laccases, nucleic acids encoding them

    2010-4-21 · Laccases, nucleic acids encoding them and methods for making and using them . Apr 21, 2010 - Verenium Corporation. The invention provides laccases, polynucleotides encoding these enzymes, the methods of making and using these polynucleotides and polypeptides. Latest …

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  • blue to a PH 5, I have been able to - JSTOR

    stain. Red staining granules in the cytoplasm of nerve cells were first described by Held,13 in 1895, by his erythrosin (iodo-eosin) methylene blue method. He termed them neurosomes. I have obtained satisfactory results from using erythrosin to stain Negri bodies. The Negri body gives a …

    Get Price
  • Proceedings of the Food and Drug Administration

    2019-5-29 · On November 29, 2018, experts in the field of infectious diseases, pathogen reduction technologies (PRTs) and other participants from blood centers, academia, and industry gathered at the Food and Drug Administration (FDA) White Oak Campus in Silver Spring, Maryland, for a 2-day public workshop entitled “Pathogen Reduction Technologies for Blood Safety.”

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  • Emerging translation strategies during virus–host ...

    2020-8-5 · Using the ribopuromycylation method to visualize subcellular translation activity, it has been reported that the virus factory of vaccinia virus and reovirus possess elevated translation activity compared to the surrounding cytoplasm (David et al., 2011; Desmet, Anguish, & Parker, 2014).

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  • Biosensors | Free Full-Text | Nanobioengineered

    Recent advancement has been accomplished in the field of biosensors through the modification of cellulose as a nano-engineered matrix material. To date, various techniques have been reported to develop cellulose-based matrices for fabricating different types of biosensors. Trends of involving cellulosic materials in paper-based multiplexing devices and microfluidic analytical technologies have ...

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  • Pseudorabies virus infection inhibits stress granules ...

    2020-8-1 · Pseudorabies virus (PRV) is one of the most notorious pathogens in the global pig industry. During infection, viruses may evolve various strategies, such as modulating stress granules (SGs) formation, to create an optimal surroundings for viral replication. However, the interplay between PRV infection and SGs formation remains largely unknown.

    Get Price
  • Function of Torsin AAA+ ATPases in Pseudorabies

    2020-3-15 · 2018年发表SCI论文-华中农业大学农业微生物学国家重点实验室. 1. Lin Da#, Hong Ping#, Zhang Siheng, Xu Weize, Jamal M, Yan Keji, Lei Yingying, Li Liang, Ruan Yijun, Fu Zhen F., Li Guoliang*, Cao Gang*, Digestion-ligation-only Hi-C is an efficient and cost-effective method for chromosome conformation capture, Nat Genet, 2018,50 (5):754. 2.

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  • Golgi staining-like retrograde labeling of brain circuits ...

    Cells were transfected with 1 µg of plasmid DNA using polyethylenimine (PEI) , and harvested 24 h post transfection by scraping into the medium, pelleted, washed twice with phosphate-buffered saline (PBS) and lysed in SDS-containing sample buffer (0.13 M Tris-HCl, pH 6.8; 4% SDS; 20% glycerin; 0.01% bromophenol blue; 10% 2-mercaptoethanol). Here we used PEI transfection, instead of calcium phosphate-precipitation method…

    Get Price
  • Quantitative electron microscope studies on the

    2020-10-1 · The gold standard to detect the fine details of neuronal dendritic arborization is still the silver staining technique that has been used by Cajal, called Golgi metallic impregnation method. This method or its updates (Rapid Golgi staining, Golgi-Cox method) allows staining a limited number of neurons in their entirety, in a still unexplained random manner.

    Get Price
  • Detection of Prion Protein in Formalin-Fixed Brain by ...

    The appearance of the fine-grained aggre- gates appears to be related to the growth of virus in the nuclei. The aggregates resemble those found in cells infected with other viruses including varicella (Tournier et al., 1957), polyoma (Bernhard et al., 1959), EMC (Hinz et al., 1962), SV40 (Gran- boulan et al., 1963), and pseudorabies (Felluga, 1963).

    Get Price
  • A soluble form of human nectin-2 impairs exocrine ...

    2018-2-23 · ing. 1 This novel method of antigen enhancement, termed hydrated autoclaving, was originally devised to improve the immunoreactivity of certain brain proteins. 10 Hydrated au-toclaving has not been used to detect other antigens. How-ever, antigen enhancement is believed to result from heat denaturation of the protein, and the same mechanism has

    Get Price
  • Progress and Perspective of Antiviral Protective

    2016-3-1 · Transgenic mice expressing hNectin-2Ig were generated as described previously , by microinjection method of the transgenic fragment containing the CAG promoter (cytomegalovirus IE enhancer and chicken ß-actin promoter), the hNectin-2Ig gene, and the rabbit ß-globin polyA signal. To identify transgenic founders, genomic DNA was prepared by using the FTA elute micro cards (Whatman) and PCR was performed using …

    Get Price
  • The development of antibodies in rabbits and cattle ...

    2020-6-23 · The average particle size of Ag NPs is an important factor that affects its antiviral ability. For example, Krzyzowska et al. report the synthesis of Ag NPs with an average particle size of 33 nm, prepared by chemical reduction method, using tannic acid as surface ligands. This Ag NPs couls effectively control the herpes simplex virus type 2 (HSV-2) infection in mice by inhibiting the adhesion …

    Get Price
  • CPC - Compilation of Changes to the Scheme

    2020-5-15 · • this method of classification is applied only when a note after the group providing for the polymer explicitly indicates which symbols of C08F 4/00 may be used for forming the C-set. 2. When classifying in group C08F 4/00, the type of catalyst can be further indexed by using indexing codes chosen from C08F 2410/00, C08F 2420/00 or their ...

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  • Erythrocytenmorphologische

    Hino, S., and N. Furusawa: On increasing the speed of hematological tests with special references to the hematocrit method with capillaries using a high-speed centrifuge. Clin. …

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  • Production of Recombinant Porcine Interferon alpha

    2010-10-20 · Interferons (IFNs) are involved in the pathogenesis and recovery of viral and other infectious diseases. Recombinant IFNs have been used as anti-infectious agents exhibiting a broad range of antiviral and immunomodulatory properties in both human and domestic animals. In this report, we describe a highly efficient and economical approach to purify porcine IFN alpha (PoIFNα) using ...

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  • Frontiers | Direct RNA Sequencing for the Study of ...

    2020-4-28 · It has been known for a few decades that transcripts can be marked by dozens of different modifications. Yet, we are just at the beginning of charting these marks and understanding their functional impact. High-quality methods were developed for the profiling of some of these marks, and approaches to finely study their impact on specific phases of the RNA life-cycle are available, including ...

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  • Frontiers | Exogenous Fecal Microbiota

    Hiroyuki Imai, Soichiro Tsuda, Tokuko Iwamori, Kiyoshi Kano, Ken Takeshi Kusakabe, Etsuro Ono, Establishment of a novel method for the production of chimeric mouse embryos using water-in-oil droplets., Experimental Animals, 70, 1, 84-90, 2021.03. 3.

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  • Kyushu University [Etsuro Ono (Professor) Faculty of ...

    2001-5-8 · Silvia Curado, Didier Y R Stainier, Ryan M Anderson, Nitroreductase-mediated cell/tissue ablation in zebrafish: a spatially and temporally controlled ablation method with applications in developmental and regeneration studies, Nature Protocols, 10.1038/nprot.2008.58, 3, …

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  • Frontiers | Expression Pattern Analysis of Antiviral

    Birbeck granules, char- >98% of the events), two subpopulations represented E.M. Bautista et al. Veterinary Immunology and Immunopathology 88 (2002) 131±148 137 Fig. 2. Skin-derived low-density cells have morphological and ultrastuctural characteristic of dendritic cells.

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  • Inducible ablation of astrocytes shows that these cells ...

    Many viruses that are pathogenic for humans, including HIV-1, herpes simplex virus and measles, can disseminate by moving directly from cell to cell. Quentin Sattentau discusses the mechanisms of ...

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  • Molecular pathways linking adipose innervation to

    Dual pseudorabies virus tracing of ... using a whole-tissue clearing method that permits visualization of fluorescently labelled ... NPY is located in cytosolic granules in the terminals of ...

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  • Production of Recombinant Porcine Interferon alpha

    2010-10-20 · Interferons (IFNs) are involved in the pathogenesis and recovery of viral and other infectious diseases. Recombinant IFNs have been used as anti-infectious agents exhibiting a broad range of antiviral and immunomodulatory properties in both human and domestic animals. In this report, we describe a highly efficient and economical approach to purify porcine IFN alpha (PoIFNα) using ...

    Get Price
  • Frontiers | Expression Pattern Analysis of Antiviral

    TRANSFERASES AND OXIDOREDUCTASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING. THEM. REFERENCE TO SEQUENCE LISTING SUBMITTED VIA EFS-WEB This application was filed electronically via the USPTO EFS-WEB server, as authorized and set forth in MPEP §1730 II.B.2.(a)(A), and this electronic filing includes an electronically submitted …

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  • WO 2009/088949 A1 - Transferases And

    In another embodiment, the invention provides methods for drilling or oil and gas well washing and/or a fracturing method using mixtures ('cocktails') of enzymes; and in one aspect, the mixture ('cocktail') comprises polymer-degrading enzymes, and optionally at least one polymer-degrading enzyme is a lignin degrading enzyme, a lignin peroxidase ...

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  • WO 2009/020459 A2 - Glucanases, Nucleic Acids

    A method of preparation of template DNA for PCR was established using the lysis buffer (1 × PCR reaction buffer supplemented with proteinase K at a concentration of 40 μg/ml) developed by the authors.

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  • Primate defensins | Nature Reviews Microbiology

    [Key physical parameters of hawthorn leaf granules by stepwise regression analysis method]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica. 42: 1894-1900. PMID 29090548 : 0.72: 2017: Shi X, Ba R, You H, Jiang Q, Huang J, Mao J, Han L, Zhang S, Zhuang Q, Yu X, Wang L, Wang Y, Li D, Zhu W, Zhang Y ...

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  • Experimental Animals - JST

    2020-2-27 · Cationic host defence peptides (CHDP), also known as antimicrobial peptides, are naturally occurring peptides that can combat infections through their direct microbicidal properties and/or by ...

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  • Molecular pathways linking adipose innervation to

    Dual pseudorabies virus tracing of ... using a whole-tissue clearing method that permits visualization of fluorescently labelled ... NPY is located in cytosolic granules in the terminals of ...

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  • SynapticIntegrationofAdult-GeneratedOlfactoryBulb ...

    2007-10-15 · transport of pseudorabies virus from mitral cells into adult-generated PG cells. Similarly, PG cells beginning at 24 d appear ... cence from lipofuscin granules, sections were stained with 1% Sudan black in 70% methanol for 5 min, cleared in 70% ethanol, and rinsed in ... using 40 or 63 oil-immersion objectives, and up to 3.24 ...

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  • CNS sympathetic outflow neurons to white fat that

    2001-8-1 · The origins of the sympathetic outflow from brain to WAT have been defined using the transneuronal retrograde viral tract tracer, the pseudorabies virus [PRV ]. Injections of PRV into WAT pads result in labeling of a hierarchical chain of functionally connected neurons that include all …

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  • WO 2009/088949 A1 - Transferases And

    TRANSFERASES AND OXIDOREDUCTASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING. THEM. REFERENCE TO SEQUENCE LISTING SUBMITTED VIA EFS-WEB This application was filed electronically via the USPTO EFS-WEB server, as authorized and set forth in MPEP §1730 II.B.2.(a)(A), and this electronic filing includes an electronically submitted …

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  • Interaction of Vibrio cholerae enterotoxin with cell ...

    2020-12-1 · Detection of protein mediated glycosphingolipid clustering by the use of resonance energy transfer between fluorescent labelled lipids. A method established by applying the system ganglioside GM1 and cholera toxin B subunit. Chemistry and Physics of Lipids 1992, 62 (3) , 269-280.

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  • Antimicrobial host defence peptides: functions and ...

    2020-2-27 · Cationic host defence peptides (CHDP), also known as antimicrobial peptides, are naturally occurring peptides that can combat infections through their direct microbicidal properties and/or by ...

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  • Journal of Veterinary Medical Science - JST

    Fine Structure of Atrial Natriuretic Peptide(ANP)-Granules in the Atrial Cardiocytes in the Pig, Cattle and Horse Hiroharu MIFUNE, Syusaku SUZUKI, Yasutaka NODA, Shiro MOHRI, Koshi MOCHIZUKI Released: February 15, 2008

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  • Copper-binding anticancer peptides from the piscidin ...

    2021-6-16 · Using oriented CD, oriented-sample solid-state NMR 57,58,79, ND 56, and extensive all-atom MD simulations 56,57,108,109, we found that P1 and …

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  • Nanotechnology - IOPscience

    2021-7-2 · Vol 2, 1991. Vol 1, 1990. Focus issues. Focus on Topological Science and Technologies in Nano-Structures Focus on 2D Materials for Microelectronic Devices and Nanoscale Heat Dissipation Focus on 3D Micro/Nano Structures Based on Atomically Thin 2D Materials Focus on Advances in Eco-Friendly Nanomaterials and Nanotechnologies: Design, Processing ...

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  • Veterinary Pathology Online - Medirabbit

    2010-11-24 · formed using rabbit antisera against HSV-I and HSV-2 (Dako, Glostrup, Denmark). Incubation with the primary an- tibodies was followed by the peroxidase—antiperoxidase technique with diaminobenzidine as chromogen. Both anti- sera strongly labeled all cells containing 11B. In neurons, frequently nucleus and cytoplasm were homogeneously stained.

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  • Bilateral Bulbospinal Projections to Pudendal

    Pseudorabies virus injections. Approximately 104 h (4.75 days) prior to euthanasia, unilateral injections of the attenuated Bartha strain (6×10 8 pfu/mL) of PRV (courtesy of Dr. L.W. Enquist) were made into the ventral head of the bulbospongiosus muscle (BSM). We found in our pilot studies, as have others, that the attenuated Bartha strain infects a few medullary neurons as early as 4 days ...

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  • The role of oxytocin in regulation of appetitive

    However, using a much shorter period of exposure to this 60% HFD, 10 days, from PND 20 to PND 29 in male Sprague Dawley rats, significantly lower OXT levels (measured by ELISA) were found in the infralimbic medial prefrontal cortex, compared to chow-fed controls . Note that there were no significant BW differences between the two groups in this ...

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  • Experimental Animals - jstage.jst.go.jp

    Effect of Mild Stress in Mice Latently Infected Pseudorabies Virus Seiichi TANAKA, Kazuaki MANNEN. Released: October 22, 2003. P383-386 Abstract; Full Text PDF[19K] ...

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  • Content Posted in 2020 | South Dakota State

    A New Method for Anticipating Changes in Investment Market Conditions, Wookjae Heo and John E. Grable. PDF. A New Method for Biomechanical Data Acquisition in Remote Laboratory Delivery During the COVID-19 Pandemic, Ahmed M. Sayed. PDF. A New Method for the Rapid Isolation of Streptococcus Bovis from Water, Jeffry F. Kohlhoff. PDF

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  • BAXTER CONFIDENTIAL PROOFREADING

    2019-2-22 · The Gelatin Matrix consists of cross-linked gelatin granules and is provided sterile and non-pyrogenic in a standard disposable syringe. The Thrombin (Human) is a sterile, non-pyrogenic, freeze-dried, vapor-heated and solvent detergent treated powder preparation made from pooled human plasma. The Sodium Chloride Solution is a sterile,

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  • Herpesviral replication compartments move and

    2011-5-24 · The role of the intranuclear movement of chromatin in gene expression is not well-understood. Herpes simplex virus forms replication compartments (RCs) in infected cell nuclei as sites of viral DNA replication and late gene transcription. These structures develop from small compartments that grow in size, move, and coalesce. Quantitative analysis of RC trajectories, derived from 4D images ...

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  • A new quantitative PCR method for the detection of ...

    2016-7-16 · Anaplasma platys is an obligate intracellular bacterium that primarily affects dogs, but it can also infect humans. Our study aimed to standardize a quantitative real-time (q)PCR method using the citrate synthase gene (gltA) as a specific target for A. platys detection in naturally infected dogs.Primers (gltA84F and gltA84R) and probe (PLATYSp) were designed to amplify an 84-bp fragment based ...

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  • Nanotechnology - IOPscience

    2021-7-2 · Vol 2, 1991. Vol 1, 1990. Focus issues. Focus on Topological Science and Technologies in Nano-Structures Focus on 2D Materials for Microelectronic Devices and Nanoscale Heat Dissipation Focus on 3D Micro/Nano Structures Based on Atomically Thin 2D Materials Focus on Advances in Eco-Friendly Nanomaterials and Nanotechnologies: Design, Processing ...

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  • Journal of Veterinary Medical Science - J-STAGE Home

    The results using the bovine blood iCa checker correlated with those using the GEM premier 3500 and i-STAT 1. Bovine blood iCa checker was “compatible” with the GEM premier 3500 and i-STAT 1 because the frequency of differences between the measurements within ± 20% of the mean were 100% (65/65, >75%) and 90.8% (59/65, >75%), respectively.

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  • Lei Zhou - Publications

    Journal of Cancer Research and Therapeutics. 14: S767-S773. PMID 30249901 DOI: 10.4103/0973-1482.187304. Zhou L, Sheng D, Deng Q, Wang D, Liu S. Development of a novel method for rapid cloning of shRNA vectors, which successfully knocked down CD44 in …

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