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xielian for epidemic diarrhea using method

  • Inactivation of porcine epidemic diarrhea virus

    2016-12-1 · Porcine epidemic diarrhea virus (PEDV) is a very contagious pathogen that has become a major economic burden for U.S. swine producers since its initial North American detection in May 2013. This coronavirus is estimated to have killed over 7 million pigs in 2013 alone with significant additional swine morbidity and …

    Get Price
  • Visual detection of porcine epidemic diarrhea

    Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method. These results indicate that this RT-PSR assay is capable of accurately detecting PEDV, and has the advantages of high specificity and sensitivity for the detection of PEDV.

    Get Price
  • Novel isolation method of porcine epidemic

    Novel isolation method of porcine epidemic diarrhea virus using suspension vero cells and immunogenicity analysis. J Clin Microbiol. 2020 Nov 11;JCM.02156-20. doi: 10.1128/JCM.02156-20.

    Get Price
  • Porcine epidemic diarrhea virus: Viral RNA

    Since 2014, porcine epidemic diarrhea virus (PEDV) has reemerged in Europe. RT-PCR methods have been described for the detection of PEDV, but none have been validated according to a norm. In this study we described the development and validation of a SYBR™ Green one-step RT-qPCR according to the French norm NF …

    Get Price
  • Visual detection of porcine epidemic diarrhea

    2019-4-15 · Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method. Wang X (1), Xu X (1), Hu W (1), Zuo K (2), Li Z (3), Kan Y (1), Yao L (4), Ji J (5), Bi Y (3). Author information: (1)Henan Provincial Engineering Laboratory of Insects Bio-reactor, China-UK-NYNU-RRes …

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  • Porcine epidemic diarrhea virus: An overview of

    2016-12-2 · Porcine epidemic diarrhea virus (PEDV) antigens can be detected in feces using antigen capture ELISAs (Callebaut et al., 1982, Carvajal et al., 1995, Rodák et al., 2005). PEDV antigen capture or sandwich ELISAs are based on the use of a pair of PEDV-specific antibodies to capture and detect virus antigens onto a …

    Get Price
  • A novel biotinylated nanobody-based blocking

    Background: Porcine epidemic diarrhea virus (PEDV), which is characterized by severe watery diarrhea, vomiting, dehydration and a high mortality rate in piglets, leads to enormous economic losses to the pork industry and remains a large challenge worldwide. Thus, a rapid and reliable method is required for …

    Get Price
  • Phylogenetic analysis of porcine epidemic

    2012-11-15 · Porcine epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic diarrhea (PED), which is characterized by severe diarrhea, dehydration and high mortality in the affected pigs. Recently, clinical outbreaks of diarrhea in suckling piglets emerged in pig-producing areas of China.

    Get Price
  • Frontiers | Development of a Novel Double

    2016-11-4 · In this study, a novel duplex nanoparticle-assisted polymerase chain reaction (nanoPCR) assay was developed to detect porcine epidemic diarrhea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV). Two pairs of primers were designed based on the conserved region within the N gene of PEDV and TGEV.

    Get Price
  • Laboratory Methods for the Diagnosis of Epidemic

    2010-10-23 · Epidemic Diarrhea The two most common types of epidemic diarrhea in developing countries are watery diarrhea caused by Vibrio cholerae serogroup O1 and bloody. diarrhea caused by Shigella dysenteriae serotype 1 (Sd1). This chapter presents an overview of these and other organisms that cause epidemic dysentery …

    Get Price
  • Visual detection of porcine epidemic diarrhea

    2019-4-15 · Porcine epidemic diarrhea virus (PEDV) is a major etiological agent of porcine epidemic diarrhea around the world. Point-of-care testing in the field is lacking owing to the requirement for a simple, robust field applicable test that does not require professional laboratory equipment. The aim of this study was to establish a novel reverse transcription …

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  • Porcine epidemic diarrhea virus: An overview of

    2016-12-2 · Porcine epidemic diarrhea virus (PEDV) is the causative agent of an acute, highly contagious, and severe enteric disease that leads to high mortality rates in suckling piglets. Therefore, accurate diagnosis of PEDV infection is critical for the implementation of control measures for the virus.

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  • Visual detection of porcine epidemic diarrhea

    2019-4-15 · 1. BMC Vet Res. 2019 Apr 15;15(1):116. doi: 10.1186/s12917-019-1851-7. Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method.

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  • Detection and phylogenetic analysis of porcine

    2017-8-28 · Porcine epidemic diarrhoea (PED) is an acute, highly contagious disease of swine caused by the PED virus (PEDV), which leads to severe vomiting and diarrhea along with dehydration and high mortality in new-born piglets [1]. PEDV belongs to the family Coronaviridae, genus Alphacoronavirus and was first …

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  • Novel Approach for Isolation and Identification of

    2017-1-21 · Porcine epidemic diarrhea (PED), which is caused by the porcine epidemic diarrhea virus (PEDV), is an acute and highly contagious enteric viral disease in nursing pigs. PED is characterized by vomiting and lethal watery diarrhea; and is a global problem, especially in many swine-producing countries [1–7].

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  • Porcine Epidemic Diarrhea Virus Induces Vero Cell ...

    2021-6-24 · Porcine Epidemic Diarrhea Virus Induces Vero Cell Apoptosis via the p53-PUMA Signaling Pathway Lin Yang 1, Chenyu Wang 1, Jinqi Shu 1, ... data are analyzed using the 2 DDCT method, and -actin was used as the house-keeping gene for internal normalization. 2.5. GO Function Annotation Analysis of Differentially …

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  • Phylogenetic analysis of porcine epidemic

    2012-11-15 · Porcine epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic diarrhea (PED), which is characterized by severe diarrhea, dehydration and high mortality in the affected pigs. Recently, clinical outbreaks of diarrhea in suckling piglets emerged in pig-producing areas of China. In this study, molecular detection of PEDV was conducted using …

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  • A simple and rapid identification method for newly

    2017-9-21 · Porcine Deltacoronavirus (PDCoV) is a newly emerged enteropathogenic coronavirus that causes diarrhea and mortality in neonatal piglets. PDCoV has spread to many countries around the world, leading to significant economic losses in the pork industry. Therefore, a rapid and sensitive method for detection of PDCoV in …

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  • A sensitive duplex nanoparticle-assisted PCR

    2016-11-4 · In this study, a novel duplex nanoparticle-assisted polymerase chain reaction (nanoPCR) assay was developed to detect porcine epidemic diarrhea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV). Two pairs of primers were designed based on the conserved region within the N gene of PEDV and …

    Get Price
  • Laboratory Methods for the Diagnosis of Epidemic

    2010-10-23 · Epidemic Diarrhea The two most common types of epidemic diarrhea in developing countries are watery diarrhea caused by Vibrio cholerae serogroup O1 and bloody. diarrhea caused by Shigella dysenteriae serotype 1 (Sd1). This chapter presents an overview of these and other organisms that cause epidemic dysentery …

    Get Price
  • Visual detection of porcine epidemic diarrhea

    2019-4-15 · Porcine epidemic diarrhea virus (PEDV) is a major etiological agent of porcine epidemic diarrhea around the world. Point-of-care testing in the field is lacking owing to the requirement for a simple, robust field applicable test that does not require professional laboratory equipment. The aim of this study was to establish a novel reverse transcription …

    Get Price
  • Outbreak of Porcine Epidemic Diarrhea in Suckling

    2011-12-22 · Phylogenetic tree constructed by using the neighbor-joining method based on the 9 porcine epidemic diarrhea virus (PEDV) sequences identified in a study of porcine epidemic diarrhea in China. Partially amplified... Top. Cite This Article. DOI: 10.3201/eid1801.111259

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  • Development of indirect enzyme-linked

    2019-11-12 · Porcine epidemic diarrhea virus (PEDV) infection is a highly contagious infectious disease causing watery diarrhea, vomiting, dehydration and high mortality rate in newborn piglets. PEDV infection can cause high economic losses in pig industry. In Japan, a PEDV outbreak occurred with high mortality from 2013 to …

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  • Construction of multiple shRNA vectors targeting

    economic losses worldwide. Porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) are the main pathogens responsible for piglet viral diarrhea. However, currently there is no specific drug available for the effective treatment of viral diarrhea. Therefore, it is necessary to seek an effective method …

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  • A new immunochromatographic assay for on-site

    BMC Veterinary Research (2019-01-01) . A new immunochromatographic assay for on-site detection of porcine epidemic diarrhea virus based on monoclonal antibodies prepared by using cell surface fluorescence immunosorbent assay

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  • A sensitive duplex nanoparticle-assisted PCR

    2016-11-4 · In this study, a novel duplex nanoparticle-assisted polymerase chain reaction (nanoPCR) assay was developed to detect porcine epidemic diarrhea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV). Two pairs of primers were designed based on the conserved region within the N gene of PEDV and …

    Get Price
  • Molecular characterization and phylogenetic

    2021-5-31 · RT-PCR method is a specific and sensitive diagnostic tool for the detection of PEDV from suspected tissue or fecal sample [17]. EM is used to detect viral particles in fecal material with diarrhea, but EM is not a sensitive technique [18]. Although, SNT is time-consuming

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  • The Carboxy Terminal Region on Spike Protein of

    2011-2-1 · Porcine epidemic diarrhea (PED) is an infectious enteric disease characterized by acute enteritis and diarrhea in pigs, and the infection is more severe in neonates [].At present, PED has been a major concern in the swine industry, particularly, in the Asia and Europe, resulting in large economic losses [2–4].The causative agent of PED is porcine epidemic diarrhea …

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  • Inactivation of porcine epidemic diarrhea virus

    2016-12-1 · Porcine epidemic diarrhea virus (PEDV) is a very contagious pathogen that has become a major economic burden for U.S. swine producers since its initial North American detection in May 2013. This coronavirus is estimated to have killed over 7 million pigs in 2013 alone with significant additional swine morbidity and …

    Get Price
  • Visual detection of porcine epidemic diarrhea

    Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method. These results indicate that this RT-PSR assay is capable of accurately detecting PEDV, and has the advantages of high specificity and sensitivity for the detection of PEDV.

    Get Price
  • Novel isolation method of porcine epidemic

    Novel isolation method of porcine epidemic diarrhea virus using suspension vero cells and immunogenicity analysis. J Clin Microbiol. 2020 Nov 11;JCM.02156-20. doi: 10.1128/JCM.02156-20.

    Get Price
  • Porcine epidemic diarrhea virus: Viral RNA

    Since 2014, porcine epidemic diarrhea virus (PEDV) has reemerged in Europe. RT-PCR methods have been described for the detection of PEDV, but none have been validated according to a norm. In this study we described the development and validation of a SYBR™ Green one-step RT-qPCR according to the French norm NF …

    Get Price
  • Visual detection of porcine epidemic diarrhea

    2019-4-15 · Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method. Wang X (1), Xu X (1), Hu W (1), Zuo K (2), Li Z (3), Kan Y (1), Yao L (4), Ji J (5), Bi Y (3). Author information: (1)Henan Provincial Engineering Laboratory of Insects Bio-reactor, China-UK-NYNU-RRes …

    Get Price
  • Porcine epidemic diarrhea virus: An overview of

    2016-12-2 · Porcine epidemic diarrhea virus (PEDV) antigens can be detected in feces using antigen capture ELISAs (Callebaut et al., 1982, Carvajal et al., 1995, Rodák et al., 2005). PEDV antigen capture or sandwich ELISAs are based on the use of a pair of PEDV-specific antibodies to capture and detect virus antigens onto a …

    Get Price
  • A novel biotinylated nanobody-based blocking

    Background: Porcine epidemic diarrhea virus (PEDV), which is characterized by severe watery diarrhea, vomiting, dehydration and a high mortality rate in piglets, leads to enormous economic losses to the pork industry and remains a large challenge worldwide. Thus, a rapid and reliable method is required for …

    Get Price
  • Phylogenetic analysis of porcine epidemic

    2012-11-15 · Porcine epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic diarrhea (PED), which is characterized by severe diarrhea, dehydration and high mortality in the affected pigs. Recently, clinical outbreaks of diarrhea in suckling piglets emerged in pig-producing areas of China.

    Get Price
  • Frontiers | Development of a Novel Double

    Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea and dehydration in sucking piglets with a high mortality rate. Here, we developed a double antibody sandwich quantitative enzyme-linked immunosorbent assay (DAS-qELISA) for detection of PEDV using a specific monoclonal antibody against PEDV N protein …

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  • A sensitive duplex nanoparticle-assisted PCR

    2016-11-4 · In this study, a novel duplex nanoparticle-assisted polymerase chain reaction (nanoPCR) assay was developed to detect porcine epidemic diarrhea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV). Two pairs of primers were designed based on the conserved region within the N gene of PEDV and TGEV.

    Get Price
  • Systemic and intestinal porcine epidemic diarrhea

    The cholera epidemic is a fatal waterborne disease causing diarrhea, dehydration, and vomiting in an individual [1, 2]. It is caused by a bacterium called Vibrio cholerae . Cholera is transmitted through ingesting contaminated drinks and food, contact with cholera patient’s feces, and touching vomit and corpse killed by the bacterium …

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  • Analysis of Cholera Epidemic Controlling Using ...

    2014-12-20 · Porcine epidemic diarrhea virus (PEDV) was detected in May 2013 for the first time in U.S. swine and has since caused significant economic loss. Obtaining a U.S. PEDV isolate that can grow efficiently in cell culture is critical for investigating pathogenesis and developing diagnostic assays and for vaccine development. An …

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  • Isolation and Characterization of Porcine Epidemic

    2020-11-11 · Porcine epidemic diarrhea virus (PEDV) causes lethal diarrhea in suckling piglets, leading to severe economic losses worldwide. There is an urgent need to find new therapeutic methods to prevent and control PEDV. Not only is there a shortage of commercial anti-PEDV drugs, but available commercial vaccines fail …

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  • Tomatidine inhibits porcine epidemic diarrhea

    2019-11-13 · Porcine epidemic diarrhea virus (PEDV) is a highly virulent coronavirus that causes severe enteric disease in pigs ().PEDV infection manifests itself as watery diarrhea and vomiting and leads to dangerous dehydration that is often fatal, particularly in neonatal piglets (2, 3).PEDV was first isolated in the United Kingdom …

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  • The 3.1-Angstrom Cryo-electron Microscopy

    2021-3-22 · Porcine epidemic diarrhea (PED), caused by PEDV, is an intestinal infectious disease characterized by vomiting, anorexia, watery diarrhea, and dehydration 9, which leads to dramatic mortality in ...

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  • Puerarin enhances intestinal function in piglets

    Porcine epidemic diarrhea virus (PEDV), the causative agent of porcine epidemic diarrhea, has caused huge economic losses in pig-producing countries. Although PEDV was long believed to replicate in the intestinal epithelium by using aminopeptidase N as a receptor, the mechanisms of PEDV infection are not fully …

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  • Tight Junction Protein Occludin Is a Porcine

    2018-5-29 · Porcine epidemic diarrhoea (PED) is an emerging disease in pigs that causes massive economic losses in the swine industry, with high mortality in suckling piglets. Early identification of PED virus (PEDV)-infected herd through surveillance or monitoring strategies is necessary for mass control of PED. However, a common …

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  • Development of pooled testing system for porcine

    2019-3-19 · Porcine epidemic diarrhea virus (PEDV), a serious swine epidemic, has been rampant in Asia since the 1990s. Despite the widespread use of PEDV vaccines, the occurrence of PEDV variants requires the discovery of new substances that inhibit these viruses. During a search for PEDV inhibitory materials from natural …

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  • Prenylated Phenolic Compounds from the Leaves

    2020-9-30 · Infectious diarrhea has high morbidity and mortality around the world. For this reason, diarrhea prediction has emerged as an important problem to prevent and control outbreaks. Numerous studies have built disease prediction models using large-scale data. However, these methods perform poorly on diarrhea data. To …

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  • Detection and phylogenetic analysis of porcine

    2016-11-25 · Porcine epidemic diarrhea (PED) has increased in severity in China since 2010. To investigate further the infectivity, genetic diversity and molecular epidemiology of its causative agent, the porcine epidemic diarrhea virus (PEDV), we assessed 129 clinical samples, which were the intestinal tissue of piglets with severe diarrhea…

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  • Development of pooled testing system for porcine

    2018-5-29 · Porcine epidemic diarrhoea (PED) is an emerging disease in pigs that causes massive economic losses in the swine industry, with high mortality in suckling piglets. Early identification of PED virus (PEDV)-infected herd through surveillance or monitoring strategies is necessary for mass control of PED. However, a common …

    Get Price
  • The construction of recombinant Lactobacillus

    2021-5-4 · Porcine epidemic diarrhea (PED) is a contagious intestinal disease caused by porcine epidemic diarrhea virus (PEDV) characterized by vomiting, diarrhea, anorexia, and dehydration, which have caused huge economic losses around the world. At present, vaccine immunity is still the most effective method to control …

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  • A new immunochromatographic assay for on-site

    2019-1-18 · Porcine epidemic diarrhea virus (PEDV) is a highly effective pathogen that can cause death of new-born piglet, resulting in big economical loss in pig farming industry. For rapid detection of PEDV, a new immunochromatographic assay (ICA) based on monoclonal antibodies (mAbs) was developed in this study. The mAbs were prepared by using PEDV positive hybridoma cells that were selected by using …

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  • The 3.1-Angstrom Cryo-electron Microscopy

    2019-11-13 · Porcine epidemic diarrhea virus (PEDV) is a highly virulent coronavirus that causes severe enteric disease in pigs ().PEDV infection manifests itself as watery diarrhea and vomiting and leads to dangerous dehydration that is often fatal, particularly in neonatal piglets (2, 3).PEDV was first isolated in the United Kingdom …

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  • Differential Detection of Transmissible

    2001-11-1 · Transmissible gastroenteritis (TGE) and porcine epidemic diarrhea (PED) are highly contagious enteric diseases of piglets. The clinical signs of these diseases are very similar and include watery, yellowish diarrhea. Thus, the effective differential detection of TGE virus and PED virus is required.

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  • Isolation and Characterization of Porcine

    2015-4-16 · Porcine deltacoronavirus (PDCoV) is a novel coronavirus that causes diarrhea in nursing piglets. Following its first detection in the United States in February 2014, additional PDCoV strains have been identified in the United States and Canada. Currently, no treatments or vaccines for PDCoV are available. In this …

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  • Intramuscular Immunization with Chemokine-

    2020-3-23 · Article Intramuscular Immunization with Chemokine-Adjuvanted Inactive Porcine Epidemic Diarrhea Virus Induces Substantial Protection in Pigs Fu-Chun Hsueh 1, Yen-Chen Chang 1,2, Chi-Fei Kao 2, Chin-Wei Hsu 1 and Hui-Wen Chang 1,2,* 1 Graduate Institute of Molecular and Comparative Pathobiology, School of …

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  • Parameter Estimation and Sensitivity Analysis of

    4.3. Estimation of Parameters. In this model we have ten parameter values to be estimated. Among these parameters, the value of , natural mortality rate is obtained from the local demography of Ethiopia. The parameter , the duration of infectiousness, is also found from dysentery diarrhea characteristics [].The initial …

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  • METHOD OF FABRICATING DETECTING AGENT

    We use carbon nano tubes (CNTs) irradiated by Co-60 to investigate the utilization of highly sensitive kit to diagnosis EBV in early stage. The Institution Review Board (IRB) of Mackay Memorial Hospital has approved the ex-vivo trail (11MMHIS089) for the diagnosis methods. Results indicated magnetic beads and CNTs has high similarity …

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  • Porcine epidemic diarrhea virus (PEDV): An

    2020-6-2 · Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus in the family Coronaviridae, causes acute diarrhea and/or vomiting, dehydration and high mortality in neonatal piglets.Two different genogroups of PEDV, S INDEL [PEDV variant containing multiple deletions and insertions in the S1 …

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  • Third Strain of Porcine Epidemic Diarrhea Virus,

    To the Editor: In April 2013, porcine epidemic diarrhea virus (PEDV) was first reported ().Since PEDV was detected, the virus has continued to spread throughout the United States and has now been reported in Mexico and Canada ().PEDV was first reported in Europe in the 1970s and was later reported in Asia during the 1980s (3,4).PEDV …

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  • Rapid differentiation of PEDV wild-type strains

    2020-6-22 · Porcine epidemic diarrhea virus (PEDV) is a serious pathogen which is characterized by severe diarrhea, vomiting, and dehydration in pigs [1, 2].PEDV is an enveloped, single-stranded, positive-sense RNA virus belonging to the alpha coronavirus genus of the Coronaviridae family [].The entire genome sequence of …

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  • Changes in cecal microbiota community of

    2019-7-16 · Diarrhea, caused by porcine epidemic diarrhea virus (PEDV), is a catastrophic gastrointestinal disease among suckling piglets, with high infectivity, morbidity, and mortality, causing huge economic losses to the pig industry. In the present study, we investigated the different microbiota from the cecal mucosa and …

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  • 'Characterizing the rapid spread of porcine

    2017-11-2 · New regulatory and consumer demands highlight the importance of animal feed as a part of our national food safety system. Porcine epidemic diarrhea virus (PEDV) is the first viral pathogen confirmed to be widely transmissible in animal food. Because the potential for viral contamination in animal food is not well …

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  • Characterizing the rapid spread of porcine epidemic ...

    2018-9-28 · farm-to-fork food safety system [1]. Recently, porcine epidemic diarrhea virus (PEDV), a swine pathogen present in other parts of the world, was identified for the first time in the United States [2, 3]. The introduction of PEDV into U.S. herds was remarkable because of the sheer magnitude of infectivity and impact on animal …

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  • RCSB PDB - 5GWZ: The structure of Porcine

    2016-9-14 · Porcine epidemic diarrhea virus (PEDV) causes high mortality in pigs. PEDV main protease (M pro ) plays an essential role in viral replication.We solved the structure of PEDV M pro complexed with peptidomimetic inhibitor N3 carrying a Michael acceptor warhead, revealing atomic level interactions. We further …

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  • Porcine epidemic diarrhea, PED ELISA kit (GREEN ...

    Porcine epidemic diarrhea, PED ELISA kit (GREEN ABIOTECHNOLOGY CO., LTD) Category: Pharmaceutical and Medical Devices Exhibitor: TUNGHAI UNIVERSITY Booth No: R1327 Characteristic . The kit is used to simultaneously detect the porcine epidemic dysentery virus (PEDV) in individual or mixed samples of pigs, such as …

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  • METHOD OF FABRICATING DETECTING AGENT

    We use carbon nano tubes (CNTs) irradiated by Co-60 to investigate the utilization of highly sensitive kit to diagnosis EBV in early stage. The Institution Review Board (IRB) of Mackay Memorial Hospital has approved the ex-vivo trail (11MMHIS089) for the diagnosis methods. Results indicated magnetic beads and CNTs has high similarity …

    Get Price
  • Porcine Epidemic Diarrhea (PED) - USDA

    2018-7-16 · Porcine Epidemic Diarrhea (PED) OVERVIEW. The porcine epidemic diarrhea (PED) virus was first recognized in the United Kingdom in 1971 and had spread throughout much of Europe and Asia by 2013. The United States Department of Agriculture’s (USDA) National Veterinary Services Laboratories (NVSL)

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  • Laboratory Processing of Fecal Specimens

    of unknown origin. Only a few pathogens cause epidemic diarrhea, although many cause sporadic diarrhea.S. dysenteriae serotype 1 and V. cholerae are the two etiologic agents responsible for most epidemic diarrhea in the developing world, contributing substantially to the burden of morbidity and mortality.S. Typ hi, the

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  • Their Antiviral Activities against Porcine Epidemic ...

    2019-7-31 · Their Antiviral Activities against Porcine Epidemic Diarrhea Virus ... cell viability was measured using the MTT method. IC50 values were calculated using SigmaPlot 10.0 software. Figure S63: The inhibitory effects of compounds 7, 15, and 16 (20 μM) on the expression

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  • Characterization and pathogenicity of Vero cell

    2019-10-28 · Biological characteristics of porcine epidemic diarrhea virus strains after 10, 64, or 120 passages. a Cytopathic effects of the three strains 12 hpi at an MOI of 0.01.b Immunofluorescence detection results for the P10, P64, and P120 strains in Vero cells infected at an MOI of 0.01 at 12 hpi.c Growth kinetics of the …

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  • Multiplex Reverse Transcription-PCR for Rapid

    2006-5-1 · Jung, K, Chae, C: 2005, RT-PCR-based dot blot hybridization for the detection and differentiation between porcine epidemic diarrhea virus and transmissible gastroenteritis virus in fecal samples using a non-radioactive digoxigenin cDNA probe.

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  • Molecular characterization and phylogenetic analysis

    2017-3-9 · Porcine epidemic diarrhea (PED) is a disease that is considered to be devastating for pig farmers with a rapid spread of infection (Wang et al., 2013; Alonso et al., 2014; Jung and Saif, 2015). The clinical symptoms of PED include severe enteritis, vomiting, and watery diarrhea, with high infectivity and lethality in piglets.

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  • Steps to Creating a Basic Epidemic Curve Using

    2011-9-13 · Steps to Creating a Basic Epidemic Curve Using Microsoft Excel 20 07 7 Step 6a – Select the Insert ribbon, then press and hold the Columns button to bring up the Columns submenu. Under 2-D column select the middle button for the two-dimensional chart option. [Note: Is not necessary to use 3-D chart options for epidemic …

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  • Survival of porcine epidemic diarrhea virus (PEDV)

    2017-9-19 · Infection with Porcine Epidemic Diarrhea Virus (PEDV) causes vomiting, diarrhea, and dehydration in young pigs. The virus made its first appearance in the U.S. in 2013, where it caused substantial neonatal mortality and economic losses in the U.S. pork industry. Based on outbreak investigations, it is hypothesized that …

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  • Method for producing target substance by

    2021-4-20 · We first used anti-pseudorabies virus (anti-PRV) cells and anti-porcine epidemic diarrhea virus (anti-PEDV) cells to verify the new method. Then, we used this method to successfully screen 5 hybridoma cell clones secreting paired antibodies against Avian influenza A (H7N9) virus within 15 days after fusion.

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  • Laboratory Processing of Fecal Specimens

    of unknown origin. Only a few pathogens cause epidemic diarrhea, although many cause sporadic diarrhea.S. dysenteriae serotype 1 and V. cholerae are the two etiologic agents responsible for most epidemic diarrhea in the developing world, contributing substantially to the burden of morbidity and mortality.S. Typ hi, the

    Get Price
  • Molecular characterization and phylogenetic analysis

    2017-3-9 · Porcine epidemic diarrhea (PED) is a disease that is considered to be devastating for pig farmers with a rapid spread of infection (Wang et al., 2013; Alonso et al., 2014; Jung and Saif, 2015). The clinical symptoms of PED include severe enteritis, vomiting, and watery diarrhea, with high infectivity and lethality in piglets.

    Get Price
  • Monoclonal Antibody-Based

    2018-2-20 · An immunohistochemistry technique was developed for the diagnosis of porcine epidemic diarrhea virus (PEDV). The technique was tested on formalin-fixed, paraffin-embedded intestinal tissues from piglets naturally infected with PEDV. Five different monoclonal antibodies (MAbs) were tested in this study.

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  • Survival of porcine epidemic diarrhea virus (PEDV)

    2017-9-19 · Infection with Porcine Epidemic Diarrhea Virus (PEDV) causes vomiting, diarrhea, and dehydration in young pigs. The virus made its first appearance in the U.S. in 2013, where it caused substantial neonatal mortality and economic losses in the U.S. pork industry. Based on outbreak investigations, it is hypothesized that …

    Get Price
  • Development of a Conventional RT-PCR Assay for

    2021-4-20 · We first used anti-pseudorabies virus (anti-PRV) cells and anti-porcine epidemic diarrhea virus (anti-PEDV) cells to verify the new method. Then, we used this method to successfully screen 5 hybridoma cell clones secreting paired antibodies against Avian influenza A (H7N9) virus within 15 days after fusion.

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  • Using experimental gaming simulations to elicit

    2018-11-6 · Porcine deltacoronavirus (PDCoV) is a newly discovered coronavirus, which belongs to the family Coronaviridae. It causes watery diarrhea, vomiting, and dehydration in newborn piglets. A sensitive RT-PCR method is urgently required to detect PDCoV infection. In this study, we developed and evaluated a conventional …

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  • Rooting out rumors, epidemics, and crime -- with

    2019-10-21 · Liu*, “Real-time analysis of quantum dot labeled single porcine epidemic diarrhea virus moving along the microtubules using single particle tracking” Scientific Reports (2019), 9(1): 1307. (IF=4.011) 6. Qi Wei#, Yangyang Li#, Javier Vargas, Jian Wang

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  • Developing a COVID-19 vaccine using inactivated

    2012-8-10 · This method can also be used to identify the origin of a spam message or a computer virus using only a limited number of sensors within the network. Trace the propagation of an epidemic

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  • WHO/CDS/CSR/EDC/99.8 Laboratory Methods for

    A. Epidemic Diarrhea The two most common types of epidemic diarrhea in developing countries are watery diarrhea caused by Vibrio cholerae serogroup O1 and bloody diarrhea caused by Shigella dysenteriae serotype 1 (Sd1). This chapter presents an overview of these and other organisms that cause epidemic dysentery and cholera.

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  • New Variants of Porcine Epidemic Diarrhea Virus,

    Updated on new introduction of porcine epidemic diarrhea virus in Thailand based on spike gene from isolates between 2008-2015. Introduction Porcine epidemic diarrhea (PED) is an acute infectious ...

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  • Updated on new introduction of porcine epidemic

    2019-4-30 · Analysis of the spike, ORF3, and nucleocapsid genes of porcine epidemic diarrhea virus circulating on Thai swine farms, 2011–2016 Supansa Tuanthap1, Sompong Vongpunsawad2, Cherdpong Phupolphan3, Ausanee Duang-in2, Suphot Wattanaphansak4, Pornchalit Assavacheep4, Apiradee …

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  • Analysis of the spike, ORF3, and nucleocapsid genes

    2018-3-2 · Porcine epidemic diarrhea (PED) caused by PED virus (PEDV) is an infectious and highly contagious viral disease of pigs. This virus is a member of the genus Coronavirus, family Coronaviridae, order Nidovirales and is closely related to the transmissible gastroenteritis virus (TGEV). The 2 viruses induce similar clinical …

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  • Multiplex reverse transcription-PCR for rapid

    2009-6-24 · Diarrhea is a major public health problem in Thailand. The Ministry of Public Health, Thailand, has been trying to monitor and control this disease for many years. The methodology and the results from this study could be useful for public health officers to develop a system to monitor and prevent diarrhea outbreaks. The objective of this study was to analyse the epidemic …

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  • Exploring spatial patterns and hotspots of diarrhea

    2012-12-9 · Epidemic C. difficile (027/BI/NAP1) has rapidly emerged in the past decade as the leading cause of antibiotic-associated diarrhea worldwide. However, the key events in evolutionary history leading ...

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  • Emergence and global spread of epidemic

    2017-10-30 · A novel method for genome-wide profiling of dynamic host-pathogen interactions using 3' end enriched RNA-seq. Sci Rep. 2017 Aug 17;7(1):8681. doi: 10.1038/s41598-017-08700-9 Hao J, Zhang Y, Fang S, Wen Z, Zhang X, Xue C, Cao Y. Evaluation of purified recombinant spike fragments for assessment of the presence of serum neutralizing antibodies against a variant strain of porcine epidemic diarrhea …

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  • Quantitative sensor and manufacturing method

    A quantitative sensor and manufacture method thereof are disclosed. This quantitative sensor has a dual-mode film bulk acoustic resonator structure to achieve desirable performances in sensitivity, accuracy and efficiency. Furthermore, this quantitative sensor serves as a fluid sensor when a fluid detection metal layer is …

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  • Some Opioid Addicts Are Using an Anti-Diarrhea

    2017-4-26 · Some addicts are chasing their high using an easier, legal but still dangerous method: by ingesting large amounts of an anti-diarrhea drug, loperamide. Some addicts are taking as many as 500 pills ...

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