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yinzhijiedukeli for pseudorabies application

  • Establishment of a Fosmid Library for Pseudorabies

    Pseudorabies virus (PRV) is a member of Alphaherpesvirinae subfamily, its neurotropism and latency infection attract the attention of many scientists. PRV tagged with a fluorescent reporter gene as a tracker has been used to analyze neuronal circuits, including anterograde and retrograde. In …

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  • Label free detection of pseudorabies virus infection in ...

    Abstract. The performance of an indirect enzyme-linked immunosorbent assay and the standard serum neutralization test for detection of antibodies to pseudorabies virus in porcine field sera were compared, using 304 sera from pseudorabies-free pigs in Canada, and 1082 and 580 swine sera from the USA and England, respectively.

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  • Evaluation of an enzyme immunoassay for detection

    A novel time-resolved fluorescence blocking lateral flow immunoassay (TRF-BLFIA) was developed for on-site differential diagnosis of pseudorabies virus (PRV)-infected and vaccinated pigs using europium nanoparticles (EuNPs)-labeled virion antigens and high titer PRV gE monoclonal antibodies (PRV gE- …

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  • Differential diagnosis of PRV-infected versus

    2020-7-14 · Establishment of a Fosmid Library for Pseudorabies Virus SC Strain and Application in Viral Neuronal Tracing.Front Microbiol .2020 Jun 11;11:1168. …

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  • Loop-mediated isothermal amplification

    2008-7-1 · A rapid, convenient and reliable pseudorabies virus (PRV) detection system was developed by using the loop-mediated isothermal amplification (LAMP) method. Six special primers were designed successfully based on the PRV DNA-binding protein (DBP) gene. The assay was optimized to amplify PRV DNA by incubation at 63 °C for 1 h.

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  • Detection of antibody to pseudorabies virus in swine

    A new method for the detection of pseudorabies antibody in swine sera was developed by use of the indirect immunoperoxidase plaque staining test. The indirect immunoperoxidase plaque staining test demonstrated serologic responses in pigs that were subcutaneously infected with pseudorabies virus. ... Moutou F, Toma B, Fortier B. Application of ...

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  • Anti-Pseudorabies Virus antibody (ab3534) | Abcam

    Relevance. Pseudorabies virus (PRV) is a member of the neurotropic viruses known as alphaherpesviruses. PRV is primarily a disease of swine which serve as a reservoir for the virus and the principal source of natural infection for a diverse range of secondary hosts, including cattle, sheep, goats, dogs, cats, and many feral species.

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  • Pseudorabies virus | Sigma-Aldrich

    Application. Available for Sale. United States Globally. Showing 1-1 of 1 result for 'Pseudorabies virus' Advanced Search. Structure Search. Relevance. Compare. 6D8 MB4. 6D8 MB4. Product Number ... Find Pseudorabies virus and related products for scientific research at MilliporeSigma

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  • Design of fusion protein for efficient preparation of ...

    2021-5-9 · Cyanovirin-N (CVN) is a cyanobacterial protein with potent neutralizing activity against enveloped virus. To achieve the economic and functional production of CVN, the CVN N-terminally fused with CL7(A mutant of the Colicin E7 Dnase) was utilized to improve the solubility and stability of CVN fusion protein (CL7-CVN). Additionally, to improve the detection limit of existing PRV diagnostic ...

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  • Establishment of a Fosmid Library for Pseudorabies

    2020-6-11 · Pseudorabies virus (PRV) is a member of Alphaherpesvirinae subfamily, its neurotropism and latency infection attract the attention of many scientists. PRV tagged with a fluorescent reporter gene as a tracker has been used to analyze neuronal circuits, including anterograde and retrograde. In this study, we used fosmid library to construct a rapid and efficient platform to generate recombinant PRV.

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  • Whole-Genome Sequence Analysis of Pseudorabies Virus ...

    2021-7-8 · viruses Article Whole-Genome Sequence Analysis of Pseudorabies Virus Clinical Isolates from Pigs in China between 2012 and 2017 in China Ruiming Hu 1,2,3,†, Leyi Wang 4,†, Qingyun Liu 1,†, Lin Hua 1, Xi Huang 1, Yue Zhang 1, Jie Fan 1, Hongjian Chen 1, Wenbo Song 1, Wan Liang 1,5, Nengshui Ding 2,6,7, Zuohua Li 8, Zhen Ding 2,3, Xibiao Tang 1, Zhong Peng 1,* and Bin Wu 1,*

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  • An Overview of Live Attenuated Recombinant

    2014-6-5 · Pseudorabies virus (PRV) is a double-stranded, DNA-based swine virus with a genome approximating 150 kb in size. PRV has many nonessential genes which can be replaced with genes encoding heterologous antigens but without deleterious effects on virus propagation. Recombinant PRVs expressing both native and foreign antigens are able to stimulate immune responses.

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  • Anti-Pseudorabies Virus antibody (ab3534) | Abcam

    Relevance. Pseudorabies virus (PRV) is a member of the neurotropic viruses known as alphaherpesviruses. PRV is primarily a disease of swine which serve as a reservoir for the virus and the principal source of natural infection for a diverse range of secondary hosts, including cattle, sheep, goats, dogs, cats, and many feral species.

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  • Enzymatic amplification of latent pseudorabies virus ...

    1991-9-1 · Application of the polymerase chain reaction (PCR) in veterinary diagnostic virology. Belák S , Ballagi-Pordány A Vet Res Commun , 17(1):55-72, 01 Jan 1993

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  • Latent pseudorabies virus infection in medulla

    2020-7-2 · Pseudorabies virus (PRV) is a major pathogen in pig husbandry and is also a risk to human well-being. Pigs with latent PRV infection carry the virus lifelong, and it can be activated under conducive conditions. This poses a very important challenge to the control of the virus and may even prevent its elimination. To investigate latent infection ...

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  • Establishment of a Fosmid Library for Pseudorabies

    2020-6-11 · The first fosmid library for pseudorabies variant strain PRV TJ has been reported in 2018 by our group (Zhou et al., 2018). In this study, a fosmid library for pseudorabies strain PRV SC was established using the same method. In this study, we got 200 fosmid DNAs that cover the whole genome of …

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  • Detection of antibody to pseudorabies virus in swine

    A new method for the detection of pseudorabies antibody in swine sera was developed by use of the indirect immunoperoxidase plaque staining test. The indirect immunoperoxidase plaque staining test demonstrated serologic responses in pigs that were subcutaneously infected with pseudorabies virus. ... Moutou F, Toma B, Fortier B. Application of ...

    Get Price
  • Latent pseudorabies virus infection in medulla

    2020-7-2 · Pseudorabies virus (PRV) is a major pathogen in pig husbandry and is also a risk to human well‐being. Pigs with latent PRV infection carry the virus lifelong, and it can be activated under conducive conditions. This poses a very important challenge to the control of the virus and may even prevent its elimination. To investigate latent ...

    Get Price
  • Human Endophthalmitis Caused By Pseudorabies

    2020-8-10 · 2020-07-14 Establishment of a Fosmid Library for Pseudorabies Virus SC Strain and Application in Viral Neuronal Tracing.Front Microbiol .2020 Jun 11;11:1168. doi: 10.3389/fmicb.2020.01168. eCollection 2020

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  • Us9 -pseudorabies virus Antibody (1A8) - DSHB

    1A8 was deposited to the DSHB by Enquist, L. Lyman, M. (DSHB Hybridoma Product 1A8) Storage and Handling Recommendations. Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended.

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  • APPLICATION FOR REGISTERED SWINE EXHIBITION/SALE

    2021-6-9 · certificate of veterinary inspection, official identification and a negative pseudorabies test within 30 days of the exhibition. THIS INFORMATION PROVIDED ON THIS APPLICATION IS TRUE AND ACCURATE UNDER PENALTY Signature of Applicant, Title, and E -Mail Address Email form to : [email protected]

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  • Enhancing expression of the pseudorabies virus ...

    ORIGINAL ARTICLE Enhancing expression of the pseudorabies virus glycoprotein E in yeast and its application in an indirect sandwich ELISA C.-Y. Wu1, C.-W. Wu2, C.-M. Liao3, M.-S. Chien4 and C. Huang1 1 Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan

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  • Enhancing expression of the pseudorabies virus ...

    2017-7-8 · Introduction. Pseudorabies virus (PRV) is an alphaherpesvirus that causes pseudorabies (also known as Aujeszky's disease) in swine, which causes severe economic losses worldwide (Mettenleiter 2000).The disease is characterized by an acute and often fatal infection in piglets, retarded growth in growing pigs and abortion in pregnant sows (Nauwynck 1997).

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  • New Technology for Pseudorabies PRV Vaccine

    Pseudorabies (Aujeszky's disease) is a viral disease caused by pseudorabies virus (PRV). PRV is mainly spread via direct contact, but may also transmit by air, water and contaminated fomites. Outbreaks of PRV in pigs are difficult to control, causing catastrophic economic losses in the swine industry.

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  • Molecular Biology of Pseudorabies Virus: Impact on ...

    SUMMARY Pseudorabies virus (PRV) is a herpesvirus of swine, a member of the Alphaherpesvirinae subfamily, and the etiological agent of Aujeszky's disease. This review describes the contributions of PRV research to herpesvirus biology, neurobiology, and viral pathogenesis by focusing on (i) the molecular biology of PRV, (ii) model systems to study PRV pathogenesis and neurovirulence, (iii) PRV ...

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  • Review Article An Overview of Live Attenuated

    2019-7-31 · Review Article An Overview of Live Attenuated Recombinant Pseudorabies Viruses for Use as Novel Vaccines BoDong, 1 DanteS.Zarlenga, 2 andXiaofengRen 1 Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University,

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  • Gene Cloning and Bioinformatics Analysis of

    The glycoprotein H(gH) gene homologue of pseudorabies virus wild strain SL(PRV-SL) was cloned by degenerate polymerase chain reaction (PCR) from PRV infected vero cells. Bioinformatics analysis was performed to predict the characteristics of gH, the results indicated that gH gene encoded a polypeptide, molecular mass of 71.95kda and comprising 687 amino acids.

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  • 'Contemporary diagnostic approaches for

    The widespread use of marker vaccines and diagnostic approaches able to differentiate infected from vaccinated animals (DIVA), has led to the elimination of PRV from domestic swine populations in many countries. Nevertheless, the PRV continues to circulate in global feral swine populations and is occasionally introduced into commercial herds. More concerning, since 2011, a highly pathogenic ...

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  • Expression of Truncated gE Gene of Pseudorabies

    With the application of gE gene deleted pseudorabies virus(PRV) vaccine worldwide, a corresponding differential diagnosis based on gE glycoprotein is needed in the project of PRV eradication. In this study, PRV gE gene without signal and transmembrane region was amplified by PCR and cloned into pGEX 6P 1, generated pGEX gE. After transformation of BL21 with pGEX gE, an expressed fusion protein ...

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  • Global stability of a pseudorabies virus model with ...

    2020-8-5 · Porcine pseudorabies infection is an acute infectious disease caused by pseudorabies virus. In this paper, we formulate a mathematical susceptible-incubating-infected-treated (SEIT) model with vertical transmission. The existence and stability of the equilibrium points of the model are characterized by the basic reproduction number Re_0.

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  • The Interacting UL31 and UL34 Gene Products of ...

    2020-12-22 · A 2.6-kbp fragment of the pseudorabies virus (PrV) genome was sequenced and shown to contain the homologues of the highly conserved herpesvirus genes UL31 and UL32. By use of a monospecific antiserum, the UL31 gene product was identified as a nuclear protein with an apparent molecular mass of 29 kDa. For functional analysis, UL31 was deleted by mutagenesis in Escherichia …

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  • CN107860921A - Pseudorabies virus antibody

    The invention discloses a kind of Pseudorabies virus antibody Quantitative detection card and application method, including detection card shell and the test strips being assemblied in detection card shell, the test strips include the plastic bottom board with pressure sensitive adhesive, paste sample pad successively on bottom plate, labeling pad, nitrocellulose filter and blotting paper, the ...

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  • For Health Requirement Information

    2021-6-3 · Pseudorabies 1. Swine originating from a Pseudorabies Stage IV or V state are exempt from the pseudorabies testing requirement. 2. Swine originating from a Pseudorabies Stage III state shall be negative to an official test for pseudorabies conducted within …

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  • The Application of hydroxy naphthol blue in loop

    The traditional diagnostic method for Pseudorabies virus (PRV) has the defect of cumbersome procedures, long period, high cost and poor specificity. Thus we built a sensitive and specific loop-mediated isothermal amplification (LAMP) method for the detection of PRV, and used hydroxy naphthol blue (HNB) as indicator to judge the reaction result.

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  • Development and application of a real-time

    This study was conducted to establish a rapid and specific quantitative PCR assay for detecting Pseudorabies virus (PRV). A pair of primers and a Taq Man probe were designed according to conserved gE gene sequences of (PRV). PCR conditions were optimized to establish a Taq Man-based real-time PCR assay for the detection of PRV. The assay was tested on samples from cell culture, suspected …

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  • Description and importance of the disease disease, also ...

    2021-7-2 · Description and importance of the disease: Aujeszky’s disease, also known as pseudorabies, is caused by an alphaherpesvirus that infects the central nervous system and other organs, such as the respiratory tract, in a variety of mammals except humans and the tailless apes.

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  • Pseudorabies | FWC

    2017-12-4 · Pseudorabies is a viral disease that causes abortions, stillbirths, and respiratory problems in pigs and death in piglets . Commercial swine production herds in the U.S. have been PRV-free since early 2003. Infections continue to occur in feral swine and in …

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  • Studies on Neuronal Tracing with Pseudorabies Virus ...

    With its abilities of trans-synaptic tracing and self-replication and wide host range,pseudorabies virus(PRV)has been applied in the field of neuroanatomy since the 1970s.Four decades of PRV application have made many advances in researches on neuronal tracing with PRV.Mechanism studies focused on investigating infection of primary neurons and tracing direction in secondary neurons,while ...

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  • UNIVERSITI PUTRA MALAYSIA ESTABLISHMENT OF A

    2019-7-10 · BASED VIRAL ASSAY FOR PSEUDORABIES VIRUS INFECTION By PARISA HONARI November 2011 Chairman: Assoc. Prof. Zeenathul Nazariah Allaudin, PhD Faculty: Veterinary Medicine Studies were conducted to establish a viral based biochip using Surface Plasmon Resonance (SPR) technology for detection of Pseudorabies virus (PrV.

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  • Location of density gradient ultracentrifugation layers

    During the process of virus density gradient ultracentrifugation purification, the centrifugal zone formed after ultracentrifugation can be located by the scattering intensity of light of different-sized particles, also known as the Tyndall effect. The pseudorabies virus (PRV) was selected and taken as the research object and discontinuous gradient sucrose as the medium, the particles in the...

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  • Methods for the Manipulation of Herpesvirus Genome

    2021-6-10 · mutants from other herpesviruses, such as pseudorabies virus (PRV) [26], VZV [27], and cytomegalovirus [28,29]. The use of ts mutants in MDV research has been limited because of the highly cell associated nature of the virus. However, a ts mutant MDV replicated poorly in vivo and failed to induce a protective immune response [30].

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  • Ivermectin, a new candidate therapeutic against SARS

    2017-1-1 · 《药物分析杂志》是中国科学技术协会主管,中国药学会主办,中国食品药品检定研究院承办,药物分析杂志编辑委员会编辑出版,国内外公开发行的专业性学术期刊。《药物分析杂志》是我国药检领域首批学科带头人创办的学术期刊,由始创于1951年的《药检工作通讯》发展而来,1981年更名为 ...

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  • Pseudorabies virus expressing enhanced green

    Pseudorabies virus expressing enhanced green fluorescent protein: A tool for in vitro electrophysiological analysis of transsynaptically labeled neurons in identified central nervous system circuits Bret N. Smith*†, Bruce W. Banfield‡§, Cynthia A. Smeraski*, Christine L. Wilcox¶, F. Edward Dudek*, Lynn W. Enquist‡, and Gary E. Pickard*i

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  • September2017 -, cdfa Pseudorabies

    2019-6-10 · Pseudorabies virus can be inactivated by cleaning anddisinfecting environmental surfaces using approved disinfectants. Theefficacy of most disinfectants is impairedby the presence of organic material, and thorough cleaningprior to their application is critical. Consult your veterinarian or technical representative of the company manufacturing

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  • Effects of physical and chemical factors on

    2020-9-25 · Pseudorabies (PR) is latent and can persist in infected sows for a long time, and thus, convalescent sows can carry the virus throughout life, causing severe economic losses to farmers and posing a tremendous challenge to PR prevention and control. Here, to investigate the biological characteristics of pseudorabies virus (PRV), a variety of physical and chemical factors were analyzed …

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  • Intranasal administration with recombinant Bacillus ...

    2019-6-7 · Pseudorabies caused by pseudorabies virus (PRV) mainly infects the swine and seriously threatens the biosafety of the other animals, including humans. Since 2011, the outbreaks of PRV mutants have caused enormous economic losses in the swine industry, and traditional vaccines cannot offer enough protection. PRV can transmit by direct contact, aerosol transmission and pollutants.

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  • Construction and Vaccine Studies on a Pseudorabies

    A pseudorabies virus (PRV) mutant with deletions in genes for glycoprotein I (gI) and glycoprotein E (gE) was constructed and evaluated as a candidate vaccine strain for its protective by virulent wildtype (wt) PRV Ea strain challenge in pigs. Transfer plasmid pIESE was constructed and co-transfected with PRV genome into IBRS-2 cells to generate a recombinant mutant PRV Ea gI-/gE- virus.

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  • Development of Real-Time Polymerase Chain Reaction

    2019-9-1 · application of the developed real-time PCR assays as a differential test for rapid and specific detection of PRV ... Pseudorabies, also known as Aujeszky’s disease, is a disease caused by the Pseudorabies virus (PRV) (officially Suid herpesvirus 1; family Herpesviridae,

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  • Passive agglutination assay for pseudorabies antibody ...

    The pseudorabies virus antigens are the product of disrupted and solubilized pseudorabies virus. The pseudorabies whole virus is first produced in tissue culture by procedures known in the art. In particular, pseudorabies virus can be grown in swine testes cells, primary pig kidney cells, a PK-15 cell line, primary rabbit kidney cells, and a ...

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  • scau.edu.cn

    2020-11-23 · Better immune efficacy triggered by the inactivated gI/gE-deleted pseudorabies virus with additional gC gene insertion in mice and weaned pigs 蒋梅 罗满林 论文 小熊猫源犬瘟热病毒株 H、F 基因的克隆及序 列分析 第一 第四 杨金金 沈永义 Human-isolated H7N9

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  • 4 Current Bioprocess Technology, Products, and ...

    2018-4-24 · Fig. 1: HOLMES is a rapid, simple and efficient method for nucleic acid detection. a An illustration of HOLMES. To detect a target DNA, specific amplification of …

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